Assignment of PDZ domain-containing protein GIPC gene (C19orf3) to human chromosome band 19p13.1 by in situ hybridization and radiation hybrid mapping.

Abstract

PDZ domain-containing proteins, by virtue of protein-protein interactions, participate in receptor clustering and intracellular signaling. PDZ domain was first identified in postsynaptic density protein, Drosophila disc large protein and zona occludens 1 (ZO-1) a tight junction protein 1 (TJP1) (Saras and Heldin, 1996; Ponting et al., 1997). GIPC is a PDZ domain containing protein identified by virtue of its binding to the carboxyl terminus of the G·i3–interacting protein GAIP (GNAI3IP) (De Vries at al., 1998a). Localization of GAIP (GNAI3IP), a regulator of G protein signaling, to clathrin-coated vesicles suggests a role for these interactions in intracellular vesicular trafficking (De Vries et al., 1998b). GIPC is highly conserved between mouse, rat and human (De Vries et al., 1998b). We isolated GIPC cDNA using yeast two-hybrid analysis by its ability to interact with the cytoplasmic tail of human melanosomal protein brown/gp75/TRP-1 (TYRP1) (Vijayasaradhi et al., 1995, and manuscript in preparation). Here we report the assignment of C19orf3, the gene encoding GIPC protein to human chromosome 19p13.1 by fluorescence in situ hybridization and more specifically linked to the genetic marker SHGC1187 by radiation hybrid mapping. Materials and methods