IgM alloantibody detection by M-MPHA, a new solid-phase assay system

Abstract

New IgM alloantibody detection system that uses anti-human IgM-antibody-sensitized magnetic particles mixed passive hemagglutination (IgM-M-MPHA) has been developed. IgM antibodies are well detected by direct agglutination using the saline tube test. Anti-IgM using IgM antibody detection is not used routinely. In our evaluation, IgM-M-MPHA showed good detection of IgM alloantibodies. When IgM-M-MPHA is used together with IgG-M-MPHA (anti-human IgG-antibody-sensitized magnetic particles mixed passive hemagglutination), alloantibodies can be distinguished easily whether it is IgG, IgM or both.