Cell-cell coupling in cultures of striatal and cortical astrocytes of the monkey Cebus apella.

Journal fur Hirnforschung Pub Date : 1999-01-01
S Gayol, T Pannicke, A Reichenbach, J A Colombo
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引用次数: 0

Abstract

Astrocytes were cultured from striatum and neocortex of fetal (embryonic day 90) monkeys (Cebus apella). The cultures grew well, and the cells retained viability after freeze-storage and thawing. The cells displayed depolarized membrane potentials (-19 and -33 mV, for striatal and cortical cells, respectively) and the vast majority of cells were dye-coupled to a mean of 7 (1-18) neighbouring cells. Cell coupling was blocked by octanol (0.25 and 0.5 mM) but was independent of high K+ (10 and 50 mM) and glutamate (500 microm). Thus, cultures of fetal primate astrocytic cells are established as a model system for studies on astroglial cell-cell coupling.

猴无鳞头纹状体和皮质星形细胞的细胞-细胞偶联培养。
从胚胎第90天的猴(Cebus apella)纹状体和新皮层中培养星形胶质细胞。培养物生长良好,细胞在冷冻和解冻后仍保持活力。细胞显示去极化膜电位(纹状体和皮质细胞分别为-19和-33 mV),绝大多数细胞与平均7(1-18)个相邻细胞染料偶联。辛醇(0.25和0.5 mM)可阻断细胞偶联,但与高K+(10和50 mM)和谷氨酸(500微米)无关。因此,胚胎灵长类星形胶质细胞的培养可以作为星形胶质细胞-细胞耦合研究的模型系统。
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