Anti-inflammatory effects of total saponins of Panax notoginseng.

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-06-01

S H Li, Y Chu

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Abstract

Aim: To study the anti-inflammatory effects of total saponins of Panax notoginseng (PnS).

Methods: Rat air-pouch acute inflammatory model was established with s.c. carrageenan (Car, 25 mg.kg-1). The protein content in exudate was measured. Micro-acid titration assay and radioimmunoassay (RIA) were applied respectively to investigate effects of PnS on phospholipase A2 (PLA2) activity and dinoprostone (Din) content in exudate. Fura-2 fluorescence technique was used to determine the intracellular free calcium concentration in neutrophils (Neu-[Ca2+]i).

Results: At 12 h, PnS 60-240 mg.kg-1 i.p. reduced Neu counts, protein content [(7.7 +/- 1.3) to (4.4 +/- 1.4) g.L-1], and Din content [(1619 +/- 391) to (883 +/- 268) ng.L-1]; inhibited the PLA2 activity in exudate [(248 +/- 42) to (157 +/- 35) kU.L-1] in a dose-dependent manner. PnS 60, 120, and 240 mg.kg-1 lowered the level of Neu-[Ca2+]i with the inhibitory rate of 9.1%, 33.2%, and 39.4%, respectively.

Conclusion: PnS has an obvious anti-inflammatory effect and its mechanisms are related to the inhibition of the Neu-[Ca2+]i level and PLA2 activity, and reduction of Din content.

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三七总皂苷的抗炎作用。

目的:研究三七总皂苷的抗炎作用。方法:用角叉菜胶(Car, 25 mg.kg-1)建立大鼠气袋急性炎症模型。测定渗出液中蛋白质含量。分别采用微酸滴定法和放射免疫法测定PnS对渗出液中磷脂酶A2 (PLA2)活性和迪诺前列石(Din)含量的影响。采用Fura-2荧光技术测定中性粒细胞(Neu-[Ca2+]i)胞内游离钙浓度。结果:12 h时，PnS 60 ~ 240 mg。蛋白质含量[(7.7 +/- 1.3)降至(4.4 +/- 1.4)g.L-1]， Din含量[(1619 +/- 391)降至(883 +/- 268)ng.L-1];抑制PLA2在渗出液中的活性[(248 +/- 42)~ (157 +/- 35)kU。L-1]呈剂量依赖性。PnS 60,120和240mg。kg-1对新-[Ca2+]i的抑制率分别为9.1%、33.2%和39.4%。结论:PnS具有明显的抗炎作用，其机制与抑制Neu-[Ca2+]i水平和PLA2活性、降低Din含量有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zhongguo yao li xue bao = Acta pharmacologica Sinica

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