Effects of opioid receptor agonists on cAMP second messenger system.

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-05-01

J G Liu, Z H Gong, B Y Qin

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Abstract

Aim: To study the mechanism underlying the difference in physical dependence potential of morphine (Mor), methadone (Met), buprenorphine (Bup), etorphine (Eto), and dihydroetorphine (DHE).

Methods: Adenylate cyclase of NG108-15 cells were used for studying the effects of different opiates on cAMP second messenger system.

Results: Bup, DHE, and Eto were distinct from Mor in naloxone-precipitated rebound response of cAMP in NG108-15 cells chronically treated with these opiates. Naloxone given to NG108-15 cells treated with Mor for 24 h produced marked rebound response of adenylate cyclase. While no such rebound response was detected when the cells were treated with Bup, DHE, and Eto for 24 h. The naloxone-induced rebound response of cAMP in chronic Met-treated NG108-15 cells was also lower than that in chronic Mor-treated NG108-15 cells. Following a prolonged exposure to Bup, DHE, and Eto for 72 h, the naloxone-induced rebound response of cAMP in these cells was still markedly lower than that in Mor-treated cells. The substitution of Mor with Bup, Met, DHE, and Eto inhibited naloxone-induced rebound response of cAMP in chronic Mor-treated NG108-15 cells.

Conclusion: There were distinct differences among these opiates in regulating cAMP second messenger system, which was related to their physical dependence potential.

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阿片受体激动剂对cAMP第二信使系统的影响。

目的:探讨吗啡(Mor)、美沙酮(Met)、丁丙诺啡(Bup)、乙托啡(Eto)、二氢乙托啡(DHE)躯体依赖电位差异的机制。方法:采用NG108-15细胞腺苷酸环化酶检测不同鸦片类药物对cAMP第二信使系统的影响。结果:n108 -15细胞长期服用这些阿片类药物后，在纳洛酮沉淀的cAMP反弹反应中，Bup、DHE和Eto与Mor不同。Mor处理NG108-15细胞24 h给予纳洛酮，腺苷酸环化酶出现明显的反弹反应。而Bup、DHE和Eto处理24 h后，细胞未见这种反弹反应。纳洛酮诱导的慢性met处理的NG108-15细胞cAMP的反弹反应也低于慢性mor处理的NG108-15细胞。在长时间暴露于Bup、DHE和Eto 72小时后，纳洛酮诱导的cAMP反弹反应在这些细胞中仍明显低于mor处理的细胞。用Bup、Met、DHE和Eto替代Mor可抑制纳洛酮诱导的慢性Mor处理的NG108-15细胞cAMP的反弹反应。结论:不同阿片类药物对cAMP第二信使系统的调节存在明显差异，这与它们的生理依赖性有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zhongguo yao li xue bao = Acta pharmacologica Sinica

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