[Effects of acetylcholine on inositol 1,4,5-triphosphate formation in cervix uteri myometrial cells of human].

H Nie, M J Wei, M Q Gao, X P Ding, Z Li
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Abstract

Aim: To detect inositol 1,4,5-triphosphate (IP3) formation of pregnant and nonpregnant human myometrial cells induced by acetylcholine (ACh).

Methods: [3H] IP3 competitive protein binding assay.

Results: Basal levels of IP3 in pregnant and nonpregnant human myometrial cells were (82 +/- 9) and (96 +/- 10) nmol.g-1 (protein), respectively (n = 6). Incubated with ACh 50 mumol.L-1 for 5 min, IP3 reached the peak levels (109 +/- 11) and (122 +/- 15) nmol.g-1 (protein), respectively (n = 6), but difference of the increments of IP3 in pregnant and nonpregnant women was not significant. The increased IP3 induced by ACh was inhibited by atropine (Atr) 1 mumol.L-1.

Conclusion: The basal IP3 level in pregnant cervix myometrial cells was higher than that in nonpregnant women. ACh increased the IP3 formation.

[乙酰胆碱对人子宫颈子宫肌膜细胞肌醇1,4,5-三磷酸形成的影响]。
目的:检测乙酰胆碱(ACh)对妊娠和非妊娠人子宫肌瘤细胞肌醇1,4,5-三磷酸(IP3)形成的影响。方法:[3H] IP3竞争性蛋白结合试验。结果:孕妇和非孕妇子宫肌瘤细胞中IP3的基础水平分别为(82 +/- 9)和(96 +/- 10)nmol。g-1(蛋白)(n = 6)。用ACh 50 mumol孵育。L-1作用5 min, IP3达到峰值(109 +/- 11)和(122 +/- 15)nmol。g-1(蛋白)差异有统计学意义(n = 6),但IP3在妊娠期与非妊娠期的增加量差异无统计学意义(p < 0.05)。乙酰胆碱引起的IP3升高被阿托品(Atr) 1mol - l -1抑制。结论:妊娠妇女子宫肌瘤细胞IP3水平明显高于非妊娠妇女。ACh增加了IP3的形成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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