Protective effects of Ginkgo biloba extract on cultured rat cardiomyocytes damaged by H2O2.

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-07-01

Y H Niu, X Y Yang, W S Bao

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Abstract

Aim: To investigate the influence of Ginkgo biloba extract (GbE) on cardiomyocytes damaged by H2O2.

Methods: Cultured rat cardiomyocytes were divided into 3 groups randomly: control group; H2O2 (2.5 mmol.L-1) group; H2O2 2.5 mmol.L-1 + GbE 150 mg.L-1 group. The cardiomyocytes were cultured in MEM (Eagle's) at 37 degrees C in the presence of 5% CO2 for 4 h. Lactate dehydrogenase (LDH) was assayed by colorimetric method. Lipid peroxidation was determined by measuring thiobarbituric acid-reactive substances. Ultrastructure was viewed under transmission electron microscope.

Results: Compared with the control group, LDH leakage and malondialdehyde (MDA) content increased in H2O2 group, LDH increased from (2166 +/- 247) U.L-1 to (5180 +/- 648) U.L-1, MDA increased from (3.5 +/- 0.2) nmol/10(6) cells to (7.2 +/- 0.4) nmol/10(6) cells (P < 0.01). The ultrastructure was damaged seriously. GbE inhibited the increase of LDH leakage and MDA content induced by H2O2. In this group, LDH decreased from (5180 +/- 648) U.L-1 to (3496 +/- 386) U.L-1, MDA decreased from (7.2 +/- 0.4) nmol/10(6) cells to (4.8 +/- 0.9) nmol/10(6) cells (P < 0.01). Ultrastructure of cells was also protected by GbE.

Conclusion: GbE protected the cardiomyocyte against H2O2 injury, the protective action was attributed to its antiperoxidative effect.

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银杏叶提取物对H2O2损伤大鼠心肌细胞的保护作用。

目的:探讨银杏叶提取物(GbE)对H2O2损伤心肌细胞的影响。方法:将培养的大鼠心肌细胞随机分为3组:对照组;H2O2 (2.5 mmol.L-1)组;H2O2 2.5 mmol。L-1 + GbE 150mg。l - 1组。心肌细胞在37℃的MEM (Eagle’s)培养基中，5% CO2存在下培养4小时。用比色法测定乳酸脱氢酶(LDH)。脂质过氧化通过测量硫代巴比妥酸反应物质来测定。透射电镜下观察超微结构。结果:与对照组比较，H2O2组LDH渗漏量和丙二醛(MDA)含量升高，LDH由(2166 +/- 247)u - l -1升高至(5180 +/- 648)u - l -1, MDA由(3.5 +/- 0.2)nmol/10(6)细胞升高至(7.2 +/- 0.4)nmol/10(6)细胞(P < 0.01)。超微结构严重受损。GbE抑制H2O2诱导的LDH渗漏和MDA含量的增加。LDH由(5180 +/- 648)μ l -1降至(3496 +/- 386)μ l -1, MDA由(7.2 +/- 0.4)nmol/10(6)个细胞降至(4.8 +/- 0.9)nmol/10(6)个细胞(P < 0.01)。GbE对细胞的超微结构也有保护作用。结论:GbE对H2O2损伤心肌细胞具有保护作用，其保护作用可能与其抗过氧化作用有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zhongguo yao li xue bao = Acta pharmacologica Sinica

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