Renal excretory function in conscious Long Evans and vasopressin deficient (Brattleboro) rats after endothelin-A receptor inhibition.

R Girchev, P Markova, D Mikhov, N Natcheff
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Abstract

All experiments were performed on conscious, freely moving male Long Evans as well as Diabetes incipidus (Brattleboro) rats (300-320 g). The endothelin-A (ETA) receptor antagonist BQ-123 (Neosystem) was administered through femoral vein cannula. Arterial blood pressure was measured trough femoral artery catheter. The bladder was cannulated for urine collection via a small suprapubic incision. After a 40 min control period BQ-123 infusion (16.4 nmol/kg/min, 25 microliters/min) was started and continued for 50 min. The effect of 32.8 nmol/kg/min BQ-123 infused in conscious Brattleboro rats was also investigated. Plasma and urine concentrations of sodium, potassium and chloride as well as osmolality were determined. Glomerular filtration rate (GFR) was estimated using the clearance of endogenous creatinine. Endothelin-A receptor inhibition by 16.4 nmol/kg/min BQ-123 infusion in conscious Long-Evans rats decreased urine flow rate by 38.4% (p < 0.02) and increased urine osmolality by 30.3% (p < 0.05). Sodium, potassium, chloride excretion did not alter. Endothelin-A receptor inhibition by 16.4 nmol/kg/min and by 32.8 nmol/kg/min BQ-123 infusion in conscious Brattleboro rats did not produce any change in urine flow rate, urine osmolality or excretion of the electrolytes studied. Endothelins acting via ETA receptors may function as an inhibitor of water reabsorption in the kidneys of conscious rats.

内皮素a受体抑制后,Long Evans和血管加压素缺乏(Brattleboro)大鼠的肾脏排泄功能
所有实验均在有意识、可自由活动的雄性Long Evans和糖尿病(Brattleboro)大鼠(300-320 g)身上进行。内皮素- a (ETA)受体拮抗剂BQ-123 (Neosystem)通过股静脉插管给药。通过股动脉导管测量动脉血压。膀胱经耻骨上小切口插管收集尿液。对照组开始注射BQ-123 (16.4 nmol/kg/min, 25微升/min),持续注射50 min,观察32.8 nmol/kg/min BQ-123对清醒Brattleboro大鼠的影响。测定血浆和尿液中钠、钾、氯的浓度以及渗透压。肾小球滤过率(GFR)通过内源性肌酐清除率来估计。16.4 nmol/kg/min BQ-123对清醒大鼠内皮素a受体的抑制作用使其尿流率降低38.4% (p < 0.02),尿渗透压升高30.3% (p < 0.05)。钠、钾、氯的排泄没有改变。16.4 nmol/kg/min和32.8 nmol/kg/min输注BQ-123对有意识的Brattleboro大鼠的内皮素- a受体的抑制作用没有引起尿流率、尿渗透压或电解质排泄的任何变化。内皮素通过ETA受体的作用可能作为清醒大鼠肾脏水重吸收的抑制剂。
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