Ribotoxins and their applications in probing the topographical structure of ribosomes.

Journal of natural toxins Pub Date : 1999-10-01
W Y Liu, Z Pu
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Abstract

Ribotoxins are a group of ribosome-inactivating proteins (RIPs) isolated mostly from plants. They inactivate ribosomes by a mechanism as RNA N-glycosidase that removes a specific adenine base from the highly conserved "S/R domain" in the largest ribosomal RNA. In this review, we introduce the major results from our laboratory in recent years on the study of the structure and function of RIPs and ribosomes: [1] Purification and characterization of the enzymatic mechanism of RIPs. Several new RIPs were purified and their RNA N-glycosidase and supercoil-dependent DNA endonuclease activities were studied. [2] The topographical structure of ribosomes. The relationship between the structure and function of ribosomes, especially of the "S/R domain" in rat 28S rRNA, were investigated by means of RIPs and other chemical probes. [3] The cytotoxicity of two RIPs to carcinoma cells. [4] Several new methods for studying RIPs and probing the structure of ribosomes were developed, i.e., radioassays for RNA N-glycosidase, glycoprotein detection by fluorescent labeling on SDS-polyacrylamide gels, and methods for small RNA sequencing.

核糖体毒素及其在探测核糖体拓扑结构中的应用。
核糖体毒素是一类主要从植物中分离出来的核糖体失活蛋白。它们通过RNA n -糖苷酶的机制使核糖体失活,这种机制可以从最大的核糖体RNA中高度保守的“S/R结构域”中去除特定的腺嘌呤碱基。本文综述了近年来本实验室在核糖核酸(RIPs)和核糖体的结构和功能研究方面的主要成果:[1]、RIPs的纯化和酶促机制的表征。纯化了几个新的核糖核酸片段,并研究了它们的RNA n -糖苷酶和超螺旋依赖性DNA内切酶活性。[2]核糖体的地理结构。利用rip和其他化学探针研究了大鼠28S rRNA中核糖体,特别是“S/R结构域”的结构与功能的关系。[3]李建平。[4]研究rip和探测核糖体结构的几种新方法被开发出来,如RNA n -糖苷酶的放射检测、sds -聚丙烯酰胺凝胶荧光标记的糖蛋白检测以及小RNA测序方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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