{"title":"Ribotoxins and their applications in probing the topographical structure of ribosomes.","authors":"W Y Liu, Z Pu","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Ribotoxins are a group of ribosome-inactivating proteins (RIPs) isolated mostly from plants. They inactivate ribosomes by a mechanism as RNA N-glycosidase that removes a specific adenine base from the highly conserved \"S/R domain\" in the largest ribosomal RNA. In this review, we introduce the major results from our laboratory in recent years on the study of the structure and function of RIPs and ribosomes: [1] Purification and characterization of the enzymatic mechanism of RIPs. Several new RIPs were purified and their RNA N-glycosidase and supercoil-dependent DNA endonuclease activities were studied. [2] The topographical structure of ribosomes. The relationship between the structure and function of ribosomes, especially of the \"S/R domain\" in rat 28S rRNA, were investigated by means of RIPs and other chemical probes. [3] The cytotoxicity of two RIPs to carcinoma cells. [4] Several new methods for studying RIPs and probing the structure of ribosomes were developed, i.e., radioassays for RNA N-glycosidase, glycoprotein detection by fluorescent labeling on SDS-polyacrylamide gels, and methods for small RNA sequencing.</p>","PeriodicalId":16437,"journal":{"name":"Journal of natural toxins","volume":"8 3","pages":"385-94"},"PeriodicalIF":0.0000,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of natural toxins","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Ribotoxins are a group of ribosome-inactivating proteins (RIPs) isolated mostly from plants. They inactivate ribosomes by a mechanism as RNA N-glycosidase that removes a specific adenine base from the highly conserved "S/R domain" in the largest ribosomal RNA. In this review, we introduce the major results from our laboratory in recent years on the study of the structure and function of RIPs and ribosomes: [1] Purification and characterization of the enzymatic mechanism of RIPs. Several new RIPs were purified and their RNA N-glycosidase and supercoil-dependent DNA endonuclease activities were studied. [2] The topographical structure of ribosomes. The relationship between the structure and function of ribosomes, especially of the "S/R domain" in rat 28S rRNA, were investigated by means of RIPs and other chemical probes. [3] The cytotoxicity of two RIPs to carcinoma cells. [4] Several new methods for studying RIPs and probing the structure of ribosomes were developed, i.e., radioassays for RNA N-glycosidase, glycoprotein detection by fluorescent labeling on SDS-polyacrylamide gels, and methods for small RNA sequencing.
核糖体毒素是一类主要从植物中分离出来的核糖体失活蛋白。它们通过RNA n -糖苷酶的机制使核糖体失活,这种机制可以从最大的核糖体RNA中高度保守的“S/R结构域”中去除特定的腺嘌呤碱基。本文综述了近年来本实验室在核糖核酸(RIPs)和核糖体的结构和功能研究方面的主要成果:[1]、RIPs的纯化和酶促机制的表征。纯化了几个新的核糖核酸片段,并研究了它们的RNA n -糖苷酶和超螺旋依赖性DNA内切酶活性。[2]核糖体的地理结构。利用rip和其他化学探针研究了大鼠28S rRNA中核糖体,特别是“S/R结构域”的结构与功能的关系。[3]李建平。[4]研究rip和探测核糖体结构的几种新方法被开发出来,如RNA n -糖苷酶的放射检测、sds -聚丙烯酰胺凝胶荧光标记的糖蛋白检测以及小RNA测序方法。