Simultaneous determination of enrofloxacin and its primary metabolite, ciprofloxacin, in plasma by HPLC with fluorescence detection

IF 1.7 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Biomedical Chromatography Pub Date : 1999-10-26 DOI:10.1002/(SICI)1099-0801(199908)13:5<350::AID-BMC889>3.0.CO;2-C

M. A. Garcia, C. Solans, J. J. Aramayona, S. Rueda, M. A. Bregante, A. de Jong

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引用次数: 38

Abstract

A simple and sensitive HPLC method has been developed for the simultaneous determination of enrofloxacin (ENR) and ciprofloxacin (CIP) in plasma. Plasma sample preparation was carried out by adding phosphate buffer (pH 7.4, 0.1 M), followed by extraction with trichloromethane. ENR, CIP and the internal standard, sarafloxacin (SAR), were separated on a reversed-phase column, and eluted with aqueous acetonitrile (80:20). The fluorescence of the column effluent was monitorized at λ_ex 338 and λ_em 425 nm. The retention times were 2.28, 3.30 and 4.40 min for CIP, ENR and SAR, respectively. The detection limit for the two compounds was 10 ng/mL. Standard curves were linearly related to concentration in the range from 1 to 1500 ng/mL. The recovery was 93% for ENR and 75% for CIP. Copyright © 1999 John Wiley & Sons, Ltd.

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荧光高效液相色谱法同时测定血浆中恩诺沙星及其主要代谢物环丙沙星的含量

建立了同时测定血浆中恩诺沙星(ENR)和环丙沙星(CIP)含量的高效液相色谱法。血浆样品制备先加入磷酸盐缓冲液(pH 7.4, 0.1 M)，然后用三氯甲烷萃取。用反相柱分离ENR、CIP和内标sarafloxacin (SAR)，用乙腈水溶液(80:20)洗脱。柱出水在λ ex338和λ em425 nm处进行荧光监测。CIP、ENR和SAR的保留时间分别为2.28、3.30和4.40 min。两种化合物的检出限均为10 ng/mL。标准曲线与浓度在1 ~ 1500ng /mL范围内呈线性相关。ENR和CIP的回收率分别为93%和75%。版权所有©1999 John Wiley &儿子,有限公司

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来源期刊

Biomedical Chromatography 生物-分析化学

CiteScore

3.60

自引率

5.60%

发文量

268

审稿时长

2.3 months

期刊介绍： Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.