Effects of bombesin on the canine gallbladder motility: in vivo and in vitro experiments.

A Shahbazian, P Raichev, R Sandeva, R Kalfin, K Milenov
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Abstract

The effects of bombesin (BM) on the canine gallbladder motility was studied under two different experimental conditions: (i) in conscious dogs with a balloon chronically implanted into the gallbladder lumen where intragallbladder pressure was recorded in mm Hg by means of a pressure transducer, and (ii) in smooth muscle preparations isolated from different regions of the gallbladder where the contractions were recorded isometrically by means of mechanoelectrical transducers. Similar to CCK8 bolus injection of. BM i.v. increased the gallbladder pressure in a dose-dependent manner. The response was characterized by a slow increase of the tone and a gradual restoration (in 4 to 8 min) of the background activity. The threshold dose and the maximum dose were 30 ng/kg and 100 ng/kg for BM and 1 ng/kg and 10 ng/kg for CCK8, respectively. Both atropin (10 to 50 micrograms/kg) and hexamethonium (0.5 to 3 mg/kg) injected i.v. 5 to 10 min before BM strongly reduced or even abolished the gallbladder response to BM. Somatostatin (1 to 2 micrograms/kg) and VIP (0.5 to 1 microgram/kg) injected 3 to 5 min before BM also exerted a strong inhibitory effect on the canine gallbladder response to BM. However BM (up to 10(-6) M) had no effect on the spontaneous or electrically-induced contractions of the canine gallbladder smooth muscle preparations. The results suggest the involvement of prejunctional cholinergic-, somatostatinergic- and VIP-ergic pathways in the bombesin-induced increase of the gallbladder pressure of conscious dogs.

熊豆素对犬胆囊运动的影响:体内和体外实验。
在两种不同的实验条件下研究了bombesin (BM)对犬胆囊运动的影响:(i)在有意识的狗的胆囊腔中长期植入球囊,通过压力传感器记录膀胱内压力(mmhg); (ii)在从胆囊不同区域分离的平滑肌制备中,通过机电传感器等距记录收缩。类似于CCK8微丸注射。静脉注射BM使胆囊压力呈剂量依赖性增加。反应的特点是音调缓慢增加,背景活动逐渐恢复(在4到8分钟内)。BM的阈剂量和最大剂量分别为30 ng/kg和100 ng/kg, CCK8的阈剂量分别为1 ng/kg和10 ng/kg。在BM前5 ~ 10min静脉注射阿托品(10 ~ 50微克/kg)和六甲铵(0.5 ~ 3mg /kg),均可显著降低甚至消除胆囊对BM的反应。BM前3 ~ 5min注射生长抑素(1 ~ 2微克/kg)和VIP(0.5 ~ 1微克/kg)对犬胆囊对BM的反应也有较强的抑制作用。然而,BM(高达10(-6)M)对犬胆囊平滑肌自发收缩或电致收缩没有影响。本研究结果提示,神经前胆碱能、生长抑素能和vip能通路参与了炸弹素诱导的有意识犬胆囊压力升高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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