Rhesus monkey (Macaca mulatta) model of Helicobacter pylori: noninvasive detection and derivation of specific-pathogen-free monkeys.

Laboratory animal science Pub Date : 1999-04-01
J V Solnick, D R Canfield, S Yang, J Parsonnet
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Abstract

Background and purpose: Development of the rhesus monkey model of Helicobacter pylori has been hampered by problems with serodetection and by the difficulty of identifying specific-pathogen (Helicobacter)-free animals. Our purpose was to determine whether detection could be improved and to determine if pathogen-free monkeys could be derived by nursery rearing.

Methods: An enzyme-linked immunoabsorbent assay (ELISA) and a [14C]urea breath test were compared to endoscopy to determine H. pylori infection status in rhesus macaques; 18 animals were hand raised in the nursery to determine whether pathogen-free animals could be selected.

Results: Helicobacter pylori infection was common in colony-raised young rhesus monkeys and was nearly universal by adulthood. Serodetection, using antigen from rhesus-derived H. pylori strains, was 95% sensitive and 94% specific. The [14C]urea breath test was 96% sensitive and 88% specific for detection of chronic Helicobacter infection in rhesus monkeys. Segregation of newborn animals within the first 24 h of life was a reliable method to obtain pathogen-free rhesus monkeys.

Conclusion: Isolation of specific-pathogen-free animals, together with better detection methods, may improve the value of the rhesus monkey model for the study of H. pylori pathogenesis, immune response, and vaccine development.

恒河猴(Macaca mulatta)幽门螺杆菌模型:无创检测和特异性无病原体猴子的衍生。
背景与目的:恒河猴幽门螺杆菌模型的发展一直受到血清检测问题和鉴定无特定病原体(幽门螺杆菌)动物困难的阻碍。我们的目的是确定是否可以提高检出率,并确定是否可以通过苗圃饲养获得无病原体猴子。方法:将酶联免疫吸附试验(ELISA)和[14C]尿素呼气试验与内窥镜检查进行比较,以确定恒河猴幽门螺杆菌感染状况;在苗圃人工饲养18只动物,以确定是否可以选择无病原体动物。结果:幽门螺杆菌感染在群体饲养的年轻恒河猴中很常见,到成年时几乎普遍存在。血清检测采用来自恒河源幽门螺杆菌菌株的抗原,敏感性为95%,特异性为94%。[14C]尿素呼气试验检测恒河猴慢性幽门螺杆菌感染的敏感性为96%,特异性为88%。分离出生后24小时内的新生动物是获得无病原体恒河猴的可靠方法。结论:分离出无特异性病原体的动物,并采用更好的检测方法,可提高恒河猴模型在研究幽门螺杆菌发病机制、免疫应答和疫苗研制方面的价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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