Insulin autoantibodies and insulin antibodies have similar binding characteristics.

B M Brooks-Worrell, D Nielson, J P Palmer
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引用次数: 27

Abstract

Type 1 diabetes is an autoimmune disease characterized by immune-mediated destruction of the pancreatic beta cells. Insulin autoantibodies (IAAs) develop in many subjects at high risk for developing type 1 diabetes prior to onset of clinical disease and exposure to exogenous insulin, whereas insulin antibodies (IAs) commonly develop in patients treated with exogenous insulin. To investigate whether the binding characteristics of IAA and IA are similar, we measured eight different binding characteristics of IAAs from 19 insulin-naive first-degree relatives of type 1 diabetes patients and compared these to the binding characteristics of IAs from 19 type 1 diabetes patients treated with exogenous insulin. IAA and IA samples were matched for percentage insulin binding. Scatchard analysis revealed that IAAs have a two-slope representation similar to IAs-that is, two populations of antibodies, a high-affinity low-capacity population and a low-affinity high-capacity population. Binding properties of the two respective populations were found to be similar for IAAs and IAs. Sipps' equation was used to generate a Hill plot and produce an index of heterogeneity, which showed further similarity between IAAs and IAs. These results suggest that the IAAs found in subjects at increased risk for type 1 diabetes, like IAs, are likely to be polyclonal in nature. The similarities between IAAs and IAs support the hypothesis that both are induced by insulin presentation to the immune system.

胰岛素自身抗体和胰岛素抗体具有相似的结合特性。
1型糖尿病是一种自身免疫性疾病,其特征是免疫介导的胰腺细胞破坏。胰岛素自身抗体(IAAs)在许多1型糖尿病高危人群在临床发病和暴露于外源性胰岛素之前产生,而胰岛素抗体(IAs)通常在接受外源性胰岛素治疗的患者中产生。为了研究IAA和IA的结合特征是否相似,我们测量了19例1型糖尿病患者未接受胰岛素治疗的一级亲属的IAAs的8种不同结合特征,并将其与19例接受外源性胰岛素治疗的1型糖尿病患者的IAs的结合特征进行了比较。IAA和IA样品的胰岛素结合百分比匹配。Scatchard分析显示,IAAs具有与ias相似的双斜率表示,即两个抗体群体,一个高亲和力低容量群体和一个低亲和力高容量群体。发现两个种群的结合特性对于IAAs和IAs是相似的。利用Sipps方程生成Hill图并生成异质性指数,进一步显示了IAAs和IAs之间的相似性。这些结果表明,在1型糖尿病风险增加的受试者中发现的IAAs,如IAs,在本质上可能是多克隆的。IAAs和IAs之间的相似性支持了两者都是由胰岛素向免疫系统呈递诱导的假设。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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