Europium (III) chelate nanoparticle-based lateral flow immunoassay strips for rapid and quantitative detection of cystatin C in serum

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography B Pub Date : 2022-04-01 DOI:10.1016/j.jchromb.2022.123133

Lun Bian , Yufeng Xiong , Hui Zhao , Huiling Guo , Zhaoyue Li , Ke Ye , Zhigao Zhang , Tiancai Liu , Yingsong Wu , Guanfeng Lin

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引用次数: 3

Abstract

The concentration of Cys C in the patient's serum can reflect the level of glomerular filtration rate and indicate the occurrence of renal failure. The establishment of a simple and rapid analytical method to quantitatively monitor the concentration of Cys C in serum could help timely detection of renal failure. In this study, we have developed an Eu (III) chelate nanoparticles based lateral flow immunoassay to fulfill real-time monitoring of Cys C concentration in serum within 15 min. This method was performed as a sandwich immunoassay with a wide detection range (0.05–10 μg/mL) and a low limit of detection (24.54 ng/mL). The intra and inter-assay coefficients of variation were 8.31–8.61% and 8.92–9.95%, respectively. Furthermore, the application of this method was evaluated by comparing the determined results with those obtained by chemiluminescence immunoassay, exhibiting a satisfactory correlation (R² = 0.9830). The developed LFIA method with satisfactory analytical performance has great potential for real-time monitoring of renal failure and self-detection for the high-risk population.

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基于铕(III)螯合物纳米颗粒的横向流动免疫测定条快速定量检测血清胱抑素C

患者血清中Cys C的浓度可以反映肾小球滤过率的水平，提示肾功能衰竭的发生。建立一种简便、快速的定量监测血清中Cys C浓度的分析方法，有助于及时发现肾功能衰竭。在本研究中，我们开发了一种基于Eu (III)螯合物纳米颗粒的横向流动免疫分析法，实现了15分钟内血清中Cys C浓度的实时监测。该方法具有检测范围宽(0.05 ~ 10 μg/mL)、检测限低(24.54 ng/mL)的夹心免疫分析法。试验内变异系数为8.31 ~ 8.61%，试验间变异系数为8.92 ~ 9.95%。并将测定结果与化学发光免疫分析法进行比较，结果显示相关性良好(R2 = 0.9830)。所建立的LFIA方法具有良好的分析性能，对肾功能衰竭的实时监测和高危人群的自我检测具有很大的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Chromatography B 医学-分析化学

CiteScore

5.60

自引率

3.30%

发文量

306

审稿时长

44 days

期刊介绍： The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. The journal also considers articles reporting developments in sample preparation, detection techniques including mass spectrometry, and data handling and analysis. Developments related to preparative separations for the isolation and purification of components of biological systems may be published, including chromatographic and electrophoretic methods, affinity separations, field flow fractionation and other preparative approaches. Applications to the analysis of biological systems and samples will be considered when the analytical science contains a significant element of novelty, e.g. a new approach to the separation of a compound, novel combination of analytical techniques, or significantly improved analytical performance.