Analysis of response development in time: an isolated organ study.

M S Jankovic, B D Beleslin, D Kouvelas, G A Paradelis
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Abstract

The routine procedure for analysing the drug action on isolated organs is the establishment of the dose-response relationship and its quantification. In the first part of the experiment, we established the dose-response relationship for acetylcholine, carbachol, betanechol and 5-hydroxytriptamine on isolated preparations of rat fundus. In the second part, we analyzed the development in time of the rat fundus response to a single concentration of each of the four agonists. The single concentrations used were slightly higher than the EC50 of the agonists eliciting an optimal response. Responses to betanechol and carbachol developed with essentially the same rate, while responses to acetylcholine and 5-hydroxytriptamine developed more rapidly and more slowly, respectively. Since the rate of response development is highly dependent on the type of the receptor the agonist activated, analysis of response development in time could be an useful adjunctive tool in the pharmacodynamic studies.

反应发展的及时分析:一项离体器官研究。
分析药物对离体器官作用的常规方法是建立剂量-反应关系并进行定量分析。在第一部分实验中,我们建立了乙酰胆碱、恰巴酚、甜菜烷酚和5-羟曲坦胺在大鼠眼底离体制剂中的剂量-反应关系。在第二部分中,我们分析了大鼠眼底对四种激动剂中每一种的单一浓度反应的时间发展。使用的单一浓度略高于激动剂的EC50,引起最佳反应。对甜菜烷酚和碳巴酚的反应速度基本相同,而对乙酰胆碱和5-羟曲坦胺的反应速度分别更快和更慢。由于反应发生的速度高度依赖于激动剂激活的受体类型,因此及时分析反应发生可能是药效学研究中有用的辅助工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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