Effects of Microcystis aeruginosa and microcystin-LR on intestinal histology, immune response, and microbial community in Litopenaeus vannamei

IF 7.3 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Pollution Pub Date : 2020-10-01 DOI:10.1016/j.envpol.2020.114774

Yafei Duan , Dalin Xiong , Yun Wang , Hongbiao Dong , Jianhua Huang , Jiasong Zhang

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引用次数: 30

Abstract

Microcystis aeruginosa (MA) is a primary hazardous cyanobacteria species in aquatic ecosystems that can produce microcystin-LR (MC-LR), which harms aquatic animals. The intestine is an important target tissue for MA and MC-LR. In this study, we investigated the effects of MA and MC-LR exposure on the intestinal microbiota variation and immune responses of Litopenaeus vannamei. Shrimp were experimentally exposed to MA and MC-LR for 72 h. The results showed that both MA and MC-LR exposure caused marked histological variation and apoptosis characteristics and increased oxidative stress in the intestine. Furthermore, the relative expression levels of antimicrobial peptide genes (ALF, Crus, Pen-3) decreased, while those of pro-inflammatory cytokines (MyD88, Rel, TNF-a), a pattern-recognition receptor (TLR4) and a mediator of apoptosis (Casp-3) increased. MA and MC-LR exposure also caused intestinal microbiota variation, including decreasing microbial diversity and disturbing microbial composition. Specifically, the relative abundance of Proteobacteria decreased in the two stress groups; that of Bacteroidetes decreased in the MA group but increased in the MC-LR group, while Tenericutes varied inversely with Bacteroidetes. Our results indicate that MA and MC-LR exposure causes intestinal histopathological and microbiota variations and induces oxidative stress and immune responses in L. vannamei. In conclusion, this study reveals the negative effects of MA and MC-LR on the intestinal health of shrimp, which should be considered in aquaculture.

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铜绿微囊藻和微囊藻毒素- lr对凡纳滨对虾肠道组织学、免疫反应和微生物群落的影响

铜绿微囊藻(Microcystis aeruginosa, MA)是水生生态系统中一种主要的有害蓝藻，可产生对水生动物有害的微囊藻毒素lr (Microcystis aeruginosa, MC-LR)。肠是MA和MC-LR的重要靶组织。在本研究中，我们研究了MA和MC-LR暴露对凡纳滨对虾肠道菌群变化和免疫反应的影响。实验结果表明，MA和MC-LR均能引起虾的组织学变化和细胞凋亡特征，并增加肠道氧化应激。抗菌肽基因(ALF、Crus、Pen-3)的相对表达水平下降，促炎因子(MyD88、Rel、TNF-a)、模式识别受体(TLR4)和细胞凋亡介质(Casp-3)的相对表达水平升高。MA和MC-LR暴露也会引起肠道微生物群的变化，包括微生物多样性的减少和微生物组成的干扰。具体来说，变形杆菌的相对丰度在两个应激组中下降;MA组Bacteroidetes降低，MC-LR组升高，Tenericutes与Bacteroidetes呈负相关。我们的研究结果表明，MA和MC-LR暴露会导致瓦纳美乳杆菌的肠道组织病理学和微生物群变化，并诱导氧化应激和免疫反应。综上所述，本研究揭示了MA和MC-LR对对虾肠道健康的负面影响，在养殖中应予以考虑。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Environmental Pollution 环境科学-环境科学

CiteScore

16.00

自引率

6.70%

发文量

2082

审稿时长

2.9 months

期刊介绍： Environmental Pollution is an international peer-reviewed journal that publishes high-quality research papers and review articles covering all aspects of environmental pollution and its impacts on ecosystems and human health. Subject areas include, but are not limited to: • Sources and occurrences of pollutants that are clearly defined and measured in environmental compartments, food and food-related items, and human bodies; • Interlinks between contaminant exposure and biological, ecological, and human health effects, including those of climate change; • Contaminants of emerging concerns (including but not limited to antibiotic resistant microorganisms or genes, microplastics/nanoplastics, electronic wastes, light, and noise) and/or their biological, ecological, or human health effects; • Laboratory and field studies on the remediation/mitigation of environmental pollution via new techniques and with clear links to biological, ecological, or human health effects; • Modeling of pollution processes, patterns, or trends that is of clear environmental and/or human health interest; • New techniques that measure and examine environmental occurrences, transport, behavior, and effects of pollutants within the environment or the laboratory, provided that they can be clearly used to address problems within regional or global environmental compartments.