[Urine protein analysis with the sodium-dodecyl-sulfate-polyacrylamide gel-electrophoresis (SDS-PAGE) in healthy cats and cats with kidney diseases].

A Meyer-Lindenberg, P Wohlsein, G Trautwein, I Nolte
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Abstract

In this investigation, the value of urine protein analysis by means of molecular-weight related sodium dodecyl-polyacryl gradient gel electrophoresis (SDS-PAGE) was examined with regard to its applicability and diagnostic significance in nephropathy in the cat. A total of 87 cats was included in the study, 30 of them that were clinically healthy served as the control group. The urine protein pattern of this group had, besides the band representing the market albumin, and additional broad band within the size of the marker transferrin. In some cases, weak bands were present within the range of the Tamm-Horsfall-protein and immunoglobulin G. Micromolecular protein bands were not demonstrable. The remaining 57 animals had a histologically proven nephropathy. Thirty-eight cats had elevated urea and/or creatinine values in the plasma (group 1), and 19 animals had values within the reference range (group 2). The urine protein pattern as evidenced by SDS-urine electrophoresis was altered in all cats with histologically proven nephropathy, and it is thus concluded that with this technique a nephropathy can be diagnosed very early and prior to changes of plasma urea and creatinine (group 2). Moreover, in most of the cases, the nephrological changes can be classified as glomerular or tubulo-interstitial (group 1 and group 2). However, it is not possible to draw exact conclusions concerning the underlying morphological changes, nor can the severity of the disease be correctly assessed.

【用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)分析健康猫和肾病猫的尿蛋白】。
本研究探讨了分子量相关十二烷基钠-聚丙烯酸酯梯度凝胶电泳(SDS-PAGE)在猫肾病诊断中的适用性和诊断意义。研究共纳入87只猫,其中30只临床健康猫作为对照组。该组的尿蛋白模式除了有代表市场白蛋白的条带外,还有在标记物转铁蛋白大小范围内的另一条宽条带。在某些情况下,在tamm - horsfall蛋白和免疫球蛋白g的范围内存在弱带,微分子蛋白带不明显。其余57只动物有组织学证实的肾病。38只猫的血浆尿素和/或肌酐值升高(第1组),19只猫的血浆尿素和/或肌酐值在参考范围内(第2组)。sds -尿电泳证实的尿蛋白模式在所有组织学证实的肾病猫中都发生了改变,因此得出结论,使用这种技术可以在血浆尿素和肌酐变化之前很早就诊断出肾病(第2组)。此外,在大多数情况下,肾脏改变可分为肾小球性或小管间质性(1组和2组)。然而,对于潜在的形态学改变无法得出确切的结论,也无法正确评估疾病的严重程度。
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