Adhesion of yeast isolated from fish gut to crude intestinal mucus of rainbow trout, Salmo gairdneri.

Abstract

Two yeast strains, Saccharomyces cerevisiae CBS 7764 and Debaryomyces hansenii Hfl CBS 8339, with a high capacity to colonize fish intestine were used in this study. The ability to adhere to crude mucus obtained from fish intestine was demonstrated for both strains. Scatchard analysis of the binding indicated a positive cooperativity for D. hansenii Hfl and absence of cooperativity for S. cerevisiae CBS 7764. In neither of the strains was adhesion extensively affected by reducing the hydrophobic interaction with p-nitrophenol, or by enhancing the hydrophobic interaction with ammonium sulfate. The adhesion was heat sensitive but resistant to trypsin treatment. We conclude that adhesion is mediated partly by specific mechanisms and partly by cell surface hydrophobicity.