{"title":"Effect of sperm-agglutinating antibodies on sperm capacitation and acrosome reaction.","authors":"H Bathla, K S Sidhu","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To study the effect of polyclonal/monospecific antisera on sperm agglutination versus capacitation as well as acrosome reaction.</p><p><strong>Materials and methods: </strong>Swim-up spermatozoa from cauda epididymides of fertile male hamsters were incubated under liquid paraffin with polyclonal/monospecific antisera obtained from immunized BALB/C mice, as well as with normal serum from control BALB/C mice, at various dilutions.</p><p><strong>Results: </strong>The anti-sperm antibodies caused a significant (P < .05) sperm agglutination of various types of dilutions below 1:1000. Both capacitation and true acrosome reaction were inhibited significantly in the spermatozoa incubated with polyclonal/monospecific antisera. Capacitation in the spermatozoa with normal serum started earlier, i.e., at 2 hours of incubation compared to 3 hours of incubation in controls.</p><p><strong>Conclusion: </strong>The data differentiate the sperm agglutinating activity from anticapacitation and antiacrosome reaction activity of antisperm antisera at 1:1000 dilution.</p>","PeriodicalId":79342,"journal":{"name":"International journal of fertility and menopausal studies","volume":"41 6","pages":"528-33"},"PeriodicalIF":0.0000,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of fertility and menopausal studies","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To study the effect of polyclonal/monospecific antisera on sperm agglutination versus capacitation as well as acrosome reaction.
Materials and methods: Swim-up spermatozoa from cauda epididymides of fertile male hamsters were incubated under liquid paraffin with polyclonal/monospecific antisera obtained from immunized BALB/C mice, as well as with normal serum from control BALB/C mice, at various dilutions.
Results: The anti-sperm antibodies caused a significant (P < .05) sperm agglutination of various types of dilutions below 1:1000. Both capacitation and true acrosome reaction were inhibited significantly in the spermatozoa incubated with polyclonal/monospecific antisera. Capacitation in the spermatozoa with normal serum started earlier, i.e., at 2 hours of incubation compared to 3 hours of incubation in controls.
Conclusion: The data differentiate the sperm agglutinating activity from anticapacitation and antiacrosome reaction activity of antisperm antisera at 1:1000 dilution.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
