Identification and mapping of a putative bombesin receptor gene on human chromosome 17q21.3+

Abstract

A mouse bombesin receptor cDNA was used as a probe to screen a human P1 genomic library. Clone HBR1 was isolated and used to localize a putative human bombesin receptor gene (HBRKS) on human chromosome 17q21.3 by fluorescent in situ hybridization (FISH). HBRKS was identified and mapped by polymerase chain reaction (PCR) amplification from a Yeast artificial chromosome (YAC) contig spanning 17q21–q23. In addition, a few candidate genes were found by exon-trapping from HBR1.