A diagnostic molecular marker for zebra mussels (Dreissena polymorpha) and potentially co-occurring bivalves: mitochondrial COI.

B S Baldwin, M Black, O Sanjur, R Gustafson, R A Lutz, R C Vrijenhoek
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Abstract

We report diagnostic differences in the nucleotide sequences of a 710-bp fragment of the mitochondrial cytochrome c oxidase subunit I gene (COI) from the zebra mussel (Dreissena polymorpha) and potentially co-occurring bivalves: the quagga mussel (Dreissena bugensis); the Asiatic clam (Corbicula fluminea), the dark false mussel (Mytilopsis leucophaeata), and the wedge clam (Rangia cuneata). The COI sequence of the deep-water "profunda" phenotype of the quagga mussel was nearly identical to that of shallow-water quagga mussels. Restriction fragment length polymorphisms (RFLPs) in this portion of COI produced species-specific differences in fragment numbers and sizes that could be used as diagnostic markers to distinguish the free-living larvae produced by these bivalves.

斑马贻贝(Dreissena polymorpha)和可能共存的双壳类的诊断分子标记:线粒体COI。
我们报告了来自斑马贻贝(Dreissena polymorpha)和可能共存的双壳类动物:斑驴贻贝(Dreissena bugensis)的线粒体细胞色素c氧化酶亚基I基因(COI)的710 bp片段的核苷酸序列的诊断差异;亚洲蛤(Corbicula fluinea),黑色假贻贝(mytillopsis leucophaeata)和楔蛤(Rangia cuneata)。斑驴贻贝的深水“深海”表型的COI序列与浅水斑驴贻贝的COI序列几乎相同。这部分COI的限制性片段长度多态性(RFLPs)产生了片段数量和大小的物种特异性差异,可以作为区分这些双壳类动物产生的自由生活幼虫的诊断标记。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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