Rat hippocampal mossy fibers contain cholecystokinin-like immunoreactivity.

4区医学 Q2 Agricultural and Biological Sciences

Anatomical Record Pub Date : 1995-12-01 DOI:10.1002/ar.1092430415

J Chandy, J P Pierce, T A Milner

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引用次数: 10

Abstract

Background: The possibility that mossy fiber endings in the rat hippocampal formation may contain cholecystokinin (CCK) was reexamined.

Methods: For this, CCK-immunoreactivity was examined by light and electron microscopy using the avidin-biotin complex method.

Results: At the light level, the topographical distribution of perikarya and processes with CCK-like immunoreactivity (CCK-LI) was similar to that previously described by others. Ultrastructural analysis of the dentate gyrus and CA3 region of the hippocampus revealed that some mossy fiber terminals contained CCK-LI most often affiliated with large, dense-core vesicles (DCV). Quantitative analysis revealed that 4-8% of the mossy terminal profiles examined (n = 350) contained CCK-labeled DCVs, which corresponded to 0.03-0.2 labeled DCVs per 100 microns2 of neuropil.

Conclusions: The presence of CCK-LI within mossy fibers in the rat suggests that there is less species variability in peptide expression in this pathway than formerly believed.

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大鼠海马苔藓纤维含有胆囊收缩素样免疫反应性。

背景:重新研究了大鼠海马结构苔藓状纤维末梢可能含有胆囊收缩素(CCK)的可能性。方法:采用亲和素-生物素复合物法，通过光镜和电镜检测cck的免疫反应性。结果:在光照水平下，核周和具有cck样免疫反应性(CCK-LI)的过程的地形分布与前人的描述相似。齿状回和海马CA3区的超微结构分析显示，一些苔藓状纤维末端含有CCK-LI，最常与大而致密的核囊泡(DCV)相关。定量分析显示，4-8%的苔藓末端分布(n = 350)含有cck标记的dcv，相当于每100 μ s神经pil含有0.03-0.2个标记的dcv。结论:大鼠苔藓纤维中CCK-LI的存在表明，该途径中肽表达的物种变异性比以前认为的要小。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Anatomical Record Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics

CiteScore

4.30

自引率

0.00%

发文量

期刊介绍： The Anatomical Record