Effect on sperm-immobilizing antibodies on the spontaneous and calcium-ionophore (A23187)-induced acrosome reaction.

International journal of fertility and menopausal studies Pub Date : 1995-07-01

I Tasdemir, M Tasdemir, J Fukuda, H Kodama, T Matsui, T Tanaka

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Abstract

Objective: To study the effect of sperm-immobilizing antibodies on spontaneous and A23187-induced acrosome reactions.

Material and methods: Swim-up spermatozoa obtained from a fertile donor were incubated with 16 sera containing sperm-immobilizing antibodies obtained from infertile women and 3 control sera obtained from healthy females. The acrosome loss, either occurring spontaneously or induced by 10-microM A23181, were examined by staining sperm with Pisum sativum agglutinin labeled with fluorescein isothiocyanate.

Results: The incidence of the spontaneous acrosome reaction of spermatozoa incubated with anti-sperm antibodies (6.25 +/- 1.4%) was significantly (P < .001) lower than that of the spermatozoa incubated with control sera (12 +/- 0.8%). The incidence of A23187-induced and inducible (incidence of induced minus spontaneous) acrosome reactions of spermatozoa incubated with sera-positive for sperm antibodies (10.4 +/- 1.3% and 4.2 +/- 1.5%) was also significantly lower (P < .001) than that of spermatozoa incubated with control sera (30.7 +/- 0.5% and 18.7 +/- 1.2%).

Conclusion: Sperm immobilizing antibodies interfere with fertilization by inhibiting the acrosome reaction.

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精子固定抗体对自发和钙离子载体(A23187)诱导顶体反应的影响。

目的:研究精子固定抗体对自发性和a23187诱导顶体反应的影响。材料和方法:将来自可育供体的游动精子与来自不育女性的含有精子固定抗体的16种血清和来自健康女性的3种对照血清孵育。用异硫氰酸荧光素标记的Pisum sativum凝集素对精子进行染色，检测顶体损失，无论顶体损失是自发发生的还是由10微米A23181诱导的。结果:抗精子抗体孵育的精子自发顶体反应发生率(6.25 +/- 1.4%)显著低于对照血清孵育的精子自发顶体反应发生率(12 +/- 0.8%)(P < 0.001)。精子抗体血清阳性精子的顶体反应发生率(10.4 +/- 1.3%和4.2 +/- 1.5%)与对照血清(30.7 +/- 0.5%和18.7 +/- 1.2%)相比，a23187诱导和诱导的顶体反应发生率(诱导负自发)显著降低(P < 0.001)。结论:精子固定抗体通过抑制顶体反应干扰受精。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International journal of fertility and menopausal studies

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