[Effect of the storage temperature and time and the test sample materials on the LDH isoenzyme activity and protein fractions in horse blood].

Abstract

The LDH-isoenzyme activities and the concentration of protein fractions did not differ among heparinized plasma and serum samples of horses. In heparinized plasma samples stored at 20-22 degrees C and -18 degrees C the LDH-isoenzyme activities and the content of the protein fractions remained constant within seven days. In contrast in heparinized blood samples the total LDH activity increased markedly after seven days of storage. This increase was shared by all LDH-isoenzymes but to different extents. While the share of LDH1, LDH2 and LDH3 decreased, the proportions of LDH4 and LDH5 increased. The concentration of the protein fractions in heparinized blood samples was not affected by storage at 20-22 degrees C for seven days.