Sex difference in free erythrocyte protoporphyrin (FEP) level. IV. Sex difference in FEP level in rabbits exposed to lead.

S Ohmori, K Harada, H Miura
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引用次数: 2

Abstract

An experimental study on Japanese White (JW) rabbits was conducted to clarify the sex difference in FEP level. Male and female rabbits (n = 14 of each sex) were used. The animals of the same sex were divided into 3 groups; i.e., control group (5% glucose solution only, n = 4), low lead dose group (Pb 0.4 mg/kg.BW, n = 5) and high lead dose group (Pb 2 mg/kg.BW, n = 5). Lead was injected intravenously twice a week for 5 wk. The following parameters were determined once a week for 5 wk: blood lead (Pb-B), FEP, Ht, Hb, erythrocyte ALA-D activity, erythrocyte pyrimidine 5'-nucleotidase (P5N), urinary coproporphyrin (CP-U), urinary delta-aminolevulinic acid (ALA-U), iron in serum (Fe-S), and serum GOT and GTP. Average levels of FEP in female rabbits were higher than those in males between the 1st and 3rd week after the lead injection in the low lead dose groups, and in the final week in the high lead dose groups. In the periods without lead injection, the average levels of FEP in the female groups were not significantly higher than the corresponding levels in the male groups in every week except in the first week in the control. However, the mean of FEP levels in all female rabbits without Pb treatment was higher than that in male rabbits (t-test). By the analysis of variance for the gains of FEP from the initial value, only the low lead dose group showed a significant sex difference (female > male); that is, the female group tended to increase when compared with the male group. Furthermore, the week when FEP began to increase in the female groups was earlier than that in the male groups in the low lead dose group. In the high lead dose group, both sexes reacted to the lead exposure from the same early week. As for the parameters of anemia, the average levels of Ht and Hb tended to be lower in females than in males, but Fe-S levels were not affected by lead in both sexes and no consistent sex difference could be observed. By lead exposure, ALA-D and P5N were inhibited, and ALA-U was increased, but these parameters showed no evident sex difference. The average levels of CP-U tended to be higher in females than in males in the administration of low lead dose and to be inversely higher in males than in females in the administration of high lead dose.(ABSTRACT TRUNCATED AT 400 WORDS)

游离红细胞原卟啉(FEP)水平的性别差异。四、接触铅家兔FEP水平的性别差异。
为了阐明FEP水平的性别差异,对日本白兔进行了实验研究。选用公、母兔,雌雄各14只。将同性动物分为3组;即对照组(仅5%葡萄糖溶液,n = 4),低铅剂量组(pb0.4 mg/kg)。体重,n = 5)和高铅剂量组(Pb 2 mg/kg)。BW, n = 5)。每周一次静脉注射铅,连续5周。每周测定1次血铅(Pb-B)、FEP、Ht、Hb、红细胞ALA-D活性、红细胞嘧啶5′-核苷酸酶(P5N)、尿同比例卟啉(CP-U)、尿δ氨基乙酰丙酸(ALA-U)、血清铁(Fe-S)、血清GOT和GTP。注射铅后1 ~ 3周,低铅剂量组母兔FEP平均水平高于公兔,高铅剂量组最后一周FEP平均水平高于公兔。在不注射铅期间,除对照组第1周外,各组雌鼠FEP的平均水平在各周内均不显著高于雄鼠。未加铅处理的母兔FEP均值均高于公兔(t检验)。对FEP较初始值的增益进行方差分析,只有低铅剂量组存在显著的性别差异(女性>男性);也就是说,与男性组相比,女性组倾向于增加。低铅剂量组雌鼠FEP开始升高的时间早于雌鼠。在高铅剂量组中,两性都从同一周开始对铅接触产生反应。在贫血参数方面,女性的平均Ht和Hb水平有低于男性的趋势,而Fe-S水平在两性中不受铅的影响,性别差异不一致。铅暴露可抑制小鼠ALA-D和P5N,增加ALA-U,但性别差异不明显。在低铅剂量下,女性的CP-U平均水平往往高于男性,在高铅剂量下,男性的CP-U平均水平往往高于女性。(摘要删节为400字)
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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