Glycosaminoglycans and Chondroitin/Dermatan Sulfate Proteoglycans in the Myocardium of a Non-Human Primate

Reza I. Bashey , Phyllis M. Sampson , Sergio A. Jimenez , Ralph Heimer
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引用次数: 5

Abstract

Lyophilized mid-wall left ventricular myocardial tissues of the long-tailed non-human primate, Macaca fascicularis, were examined for the presence of glycosaminoglycans and chondroitin/dermatan sulfate proteoglycans. Mean uronic acid concentration in all samples was 0.97 ± 0.27 μg per mg dry weight myocardium. The distribution of the glycosaminoglycans in the myocardium, determined by cellulose acetate strip electrophoresis was 62 ± 4% heparan sulfate, 20 ± 6% hyaluronan, and 16 ± 5% chondroitin/dermatan sulfate. The analysis of chondroitin/ dermatan sulfate proteoglycans, done directly on the extracts of lyophilized myocardium using agarose-acrylamide gel electrophoresis and Western blotting with monoclonal antibodies to various carbohydrate epitopes and with polyclonal antibodies to the protein core, showed the presence of biglycan and decorin. That these two and no other chondroitin/dermatan sulfates were present was established by core protein analysis using SDS PAGE and Western blotting. Quantification of chondroitin/dermatan sulfate proteoglycans uncovered high individual specific variability of the chondroitin/dermatan sulfate epitopes, but only moderate variability of biglycan and decorin core proteins. The variability of the chondroitin/dermatan sulfate epitopes is most likely related to individual specific differences in chain number, iduronate content and sulfation patterns of biglycan and decorin.

非人灵长类动物心肌中的糖胺聚糖和软骨素/硫酸皮肤蛋白聚糖
研究了长尾非人灵长类动物束状猕猴(Macaca fascicularis)左心室中壁心肌冻干组织中糖胺聚糖和软骨素/硫酸皮肤蛋白聚糖的存在。各组心肌糖醛酸平均浓度为0.97±0.27 μg / mg干重。乙酸纤维素条带电泳测定糖胺聚糖在心肌中的分布为硫酸肝素(62±4%)、透明质酸(20±6%)和硫酸软骨素(16±5%)。用琼脂糖-丙烯酰胺凝胶电泳和Western blotting对冻干心肌提取物进行软骨素/硫酸皮肤聚糖蛋白多糖的分析,发现含有多糖和decorin。采用SDS - PAGE和Western blotting对核心蛋白进行分析,证实上述两种蛋白均不存在。软骨素/硫酸皮肤聚糖蛋白聚糖的定量分析揭示了软骨素/硫酸皮肤聚糖表位的高度个体特异性变异性,而大聚糖和decorin核心蛋白只有中等变异性。软骨素/硫酸皮肤素表位的可变性很可能与大聚糖和decorin的链数、伊杜酸含量和磺化模式的个体特异性差异有关。
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