The storage of cells from different tumor lines in a mechanical freezer at -80 degrees C. Comparison to cryopreservation in liquid nitrogen.

Abstract

This paper demonstrates that it is possible to store cells from different tumor lineages in a mechanical freezer at -80 degrees C. Tumor cells cryopreserved without controlled-rate freezing in medium containing 10% dimethyl sulfoxide (DMSO) and 10% Bovine Calf Serum (BCS) for 6 months displayed similar post-thawing viability as cells cryopreserved in liquid nitrogen at -196 degrees C. We recommend this method of cryopreservation as a convenient and less expensive alternative to liquid nitrogen storage. Further studies concerning the maximum storage time at -80 degrees C and studies comparing the optimal composition of the storage medium will further define the limitations and potential of this method.