A multi-stimuli-responsive nanochannel inspired by biological disulfide bond

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2023-06-14 DOI:10.1016/j.talanta.2023.124785

Xiaofang Wang, Huiming Wang, Meining Zhang

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Abstract

Disulfide bonds exist widely in channel protein and play an essential role in matter exchange and signal transduction (e.g., rhodopsin, canonical transient receptor potential 5 (TRPC5)). The research on disulfide bond in nanochannel is significant for the cognition of their biological functions. However, the fragility of biological channel limits the in-situ study and practical application. Herein, an innovative biologically-inspired artificial nanochannel based on disulfide bond (NCDS) with excellent durability, adjustable surface property is proposed. The constructed NCDS has a multi-response to UV-light, thiol (e.g., cysteine (Cys)) or pH stimulation, and can obtain reversibility after regulation by hydrogen peroxide (H₂O₂) or H⁺. The biomimetic NCDS shows great potential in biosensor and intelligent response design. This study also shines new light to channel protein based on disulfide bond that despite the nanochannel has specificity, it will be modulated by the change of nature environment, such as UV-light and chemical microenvironment (e.g., redox state and pH), which might be the reason of some disease.

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受生物二硫键启发的多刺激响应纳米通道

二硫键广泛存在于通道蛋白中，在物质交换和信号转导中发挥重要作用(如视紫红质、典型瞬时受体电位5 (TRPC5))。纳米通道中二硫键的研究对于认识其生物学功能具有重要意义。然而，生物通道的脆弱性限制了原位研究和实际应用。本文提出了一种新型的基于二硫键(NCDS)的生物启发人工纳米通道，该通道具有优异的耐久性和可调节的表面特性。所构建的NCDS对紫外光、硫醇(如半胱氨酸(Cys))或pH刺激具有多重响应，并在过氧化氢(H2O2)或H+的调控下获得可逆性。仿生NCDS在生物传感器和智能响应设计方面显示出巨大的潜力。本研究也为基于二硫键的通道蛋白提供了新的视角，尽管纳米通道具有特异性，但它会受到自然环境的改变，如紫外线和化学微环境(如氧化还原态和pH)的调节，这可能是某些疾病发生的原因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.