Interrelation between the renal sympathetic nerve and atrial natriuretic peptide in conscious long Evans rats subjected to sodium restriction.

N Natcheff, T Avramova, L Kirkova, A Tzoncheva, O Koparanova
{"title":"Interrelation between the renal sympathetic nerve and atrial natriuretic peptide in conscious long Evans rats subjected to sodium restriction.","authors":"N Natcheff,&nbsp;T Avramova,&nbsp;L Kirkova,&nbsp;A Tzoncheva,&nbsp;O Koparanova","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The renal excretory function and plasma renin activity (PRA) were studied in conscious rats on a low sodium diet (25 mmol/kg) after atrial natriuretic peptide (ANP) infusion (100 ng/kg b.w./min) for 80 min through a catheter implanted in the right atrium. The half of the animals were with bilateral kidney denervation. The rats were housed every day from 8 a.m. to 2 p.m. in individual metabolic cages for urine collection and Na, Cl, osmolality and endogenous creatinine determination. At the last day of the experiments after the ANP infusion, blood was taken from the heart for electrolytes, endogenous creatinine and PRA. The effect of denervation was monitored by measuring of noradrenaline in kidney homogenate. The data indicated that even at low sodium diet ANP stimulates the diuresis and sodium excretion without changing the glomerular filtration rate (GFR). The kidney denervation combined with ANP infusion increased twice the diuresis and four times sodium excretion vs. the control animals. In the same time PRA was decreased by about 70%. We assume that the low sodium diet attenuates the effect of ANP in respect to the excretory function. This inhibitory effect is amplified by the renal sympathetic nerves. The decrease of PRA and possibly the increased activity of renal receptors after the denervation could explain the data obtained.</p>","PeriodicalId":7035,"journal":{"name":"Acta physiologica et pharmacologica Bulgarica","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta physiologica et pharmacologica Bulgarica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

The renal excretory function and plasma renin activity (PRA) were studied in conscious rats on a low sodium diet (25 mmol/kg) after atrial natriuretic peptide (ANP) infusion (100 ng/kg b.w./min) for 80 min through a catheter implanted in the right atrium. The half of the animals were with bilateral kidney denervation. The rats were housed every day from 8 a.m. to 2 p.m. in individual metabolic cages for urine collection and Na, Cl, osmolality and endogenous creatinine determination. At the last day of the experiments after the ANP infusion, blood was taken from the heart for electrolytes, endogenous creatinine and PRA. The effect of denervation was monitored by measuring of noradrenaline in kidney homogenate. The data indicated that even at low sodium diet ANP stimulates the diuresis and sodium excretion without changing the glomerular filtration rate (GFR). The kidney denervation combined with ANP infusion increased twice the diuresis and four times sodium excretion vs. the control animals. In the same time PRA was decreased by about 70%. We assume that the low sodium diet attenuates the effect of ANP in respect to the excretory function. This inhibitory effect is amplified by the renal sympathetic nerves. The decrease of PRA and possibly the increased activity of renal receptors after the denervation could explain the data obtained.

限钠后清醒长Evans大鼠肾交感神经与心房利钠肽的关系。
采用低钠饮食(25 mmol/kg)的清醒大鼠右心房置管灌注100 ng/kg b.w./min的心房利钠肽(ANP) 80 min后,观察其肾脏排泄功能和血浆肾素活性(PRA)的变化。半数小鼠为双侧肾去神经。每天上午8点至下午2点,将大鼠置于单独的代谢笼中收集尿液并测定Na、Cl、渗透压和内源性肌酐。在ANP输注后的实验最后一天,从心脏取血检测电解质、内源性肌酐和PRA。通过测定肾匀浆中去甲肾上腺素来监测去神经支配的效果。结果表明,即使在低钠饮食中,ANP也能刺激利尿和钠排泄,但不会改变肾小球滤过率(GFR)。与对照组相比,肾去神经联合ANP输注使利尿量增加2倍,钠排泄量增加4倍。同时,PRA降低约70%。我们假设低钠饮食在排泄功能方面减弱了ANP的作用。这种抑制作用被肾交感神经放大。去神经支配后PRA的减少和肾受体活性的可能增加可以解释所获得的数据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信