{"title":"In vitro effect of DDS on phytohaemagglutinin (PHA)--induced lymphocyte transformation.","authors":"S K Ghei, U Sengupta, K V Desikan","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>A study to find out the in vitro effect of diamino diphenyl sulphone (DDS) on phytohaemagglutinin (PHA)-induced lymphocyte transformation was carried out in three phases using a wide range of DDS concentrations. Lymphocytes from healthy volunteers were investigated. Volunteers were divided into three groups to conduct the study in three phases. In each phase in addition to 0.02 ml of PHA, 4 different concentrations of DDS were added per 10(6) lymphocytes in tissue culture system. A statistically significant depression (P less than 0.05) in the per cent of blast cell formation induced by PHA was observed in the cultures with all the concentrations of DDS except with the lowest concentration (0.01 microgram) of DDS. While the depression observed in the first and the third phase was found to be dose dependent, no significant correlation was noted between the DDS concentration and depression in PHA induced blastogenesis in the second phase. The significance of these observations are discussed.</p>","PeriodicalId":75893,"journal":{"name":"Hansenologia internationalis","volume":"5 2","pages":"112-8"},"PeriodicalIF":0.0000,"publicationDate":"1980-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hansenologia internationalis","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
A study to find out the in vitro effect of diamino diphenyl sulphone (DDS) on phytohaemagglutinin (PHA)-induced lymphocyte transformation was carried out in three phases using a wide range of DDS concentrations. Lymphocytes from healthy volunteers were investigated. Volunteers were divided into three groups to conduct the study in three phases. In each phase in addition to 0.02 ml of PHA, 4 different concentrations of DDS were added per 10(6) lymphocytes in tissue culture system. A statistically significant depression (P less than 0.05) in the per cent of blast cell formation induced by PHA was observed in the cultures with all the concentrations of DDS except with the lowest concentration (0.01 microgram) of DDS. While the depression observed in the first and the third phase was found to be dose dependent, no significant correlation was noted between the DDS concentration and depression in PHA induced blastogenesis in the second phase. The significance of these observations are discussed.
研究了二氨基二苯基砜(DDS)对植物血凝素(PHA)诱导的淋巴细胞转化的体外影响，采用了不同浓度的DDS，分三期进行。研究了健康志愿者的淋巴细胞。志愿者被分成三组，分三个阶段进行研究。在每一阶段，除0.02 ml PHA外，每10(6)个淋巴细胞加入4种不同浓度的DDS。除最低浓度(0.01微克)的DDS外，其他浓度的DDS均显著降低了PHA诱导的母细胞形成率(P < 0.05)。在第一期和第三期观察到的抑制被发现是剂量依赖性的，在第二期发现DDS浓度与PHA诱导的囊胚发生抑制之间没有显著的相关性。讨论了这些观察结果的意义。
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