[Glycoconjugate biosynthesis in the arterial wall. VIII. Separation of molecular types in microsomal sialyltransferase].

Abstract

Sialyltransferase activity of the intima-media of arterial walls is located in microsomes. The solubilization of enzyme by Triton X-100, purification by chromatography and isoelectric focusing lead to two isoenzymes (pHi 4.5 and 6.4), the second is widely predominant. Aortic sialyltransferase is sensitive to nucleotides, Mn++ dependent, and the affinity for substrate N-acetyl-neuraminic acid is 300 microM.