Effect of theophylline on binding of triiodothyronine, thyroxine, thyroxamine, tetraiodothyroacetic acid and cortisol in the cytosol of human leukocytes.

Endokrinologie Pub Date : 1982-06-01
V Felt, I Ploc
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Abstract

Inhibition constants describing competitive potency of thyroactive analogues and cortisol against labelled triiodothyronine (T3) in cytosol in human leukocytes were determined in the presence and the absence of theophylline by means of charcoal-dextran assay. L-triiodothyronine, L-thyroxine,L-thyroxamine,L-tetraiodothyroacetic acid, D-thyroxine and cortisol competed with T3 in binding sites of cytosolic proteins without theophylline addition. While theophylline addition increased the competitive potency (decreased the values of inhibition constants) of all thyroactive analogues, no such equivocal effect has been found in the case of cortisol.

茶碱对人白细胞胞浆中三碘甲状腺原氨酸、甲状腺素、甲状腺胺、四碘甲状腺乙酸和皮质醇结合的影响。
在存在和不存在茶碱的情况下,用炭-葡聚糖法测定了人白细胞细胞质中甲状腺活性类似物和皮质醇对标记三碘甲状腺原氨酸(T3)的竞争效力的抑制常数。在不添加茶碱的情况下,l -三碘甲状腺原氨酸、l -甲状腺素、l -甲状腺胺、l -四碘甲状腺乙酸、d -甲状腺素和皮质醇在胞质蛋白结合位点与T3竞争。虽然添加茶碱增加了所有甲状腺活性类似物的竞争效力(降低了抑制常数的值),但在皮质醇的情况下没有发现这种模棱两可的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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