Tetrahedral DNA nanostructure based biosensor for high-performance detection of circulating tumor DNA using all-carbon nanotube transistor

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2022-02-01 DOI:10.1016/j.bios.2021.113785

Shenhui Ma , Yaping Zhang , Qinqi Ren , Xiaofang Wang , Jiahao Zhu , Feng Yin , Zigang Li , Min Zhang

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引用次数: 18

Abstract

Adopting carbon nanotube (CNT) transistors as biosensors has been developed as a promising method for cancer biomarker detection, which has shown superior sensitivity and selectivity. However, the detection of circulating tumor DNA (ctDNA) by the CNT transistor based biosensors is still a challenge and no work has been reported. Here, direct label-free DNA detection of AKT2 gene related to triple-negative breast cancer by all-CNT thin-film transistor (TFT) biosensors incorporated with tetrahedral DNA nanostructures (TDNs) is proposed and achieved for the first time. The adoption of TDNs enables improved biosensor response for at least 35% and even as high as 98% as compared with single-stranded DNA (ssDNA) probes owing to the enhanced DNA hybridization efficiency. Influence of the TDNs’ linker length on the biosensor performance is important and has been investigated. Concentration-dependent DNA detection is achieved by the all-CNT TFT biosensors with a broad linear detection range of six orders of magnitude and a theoretical limit of detection (LOD) of 2 fM. In addition, the all-CNT TFT biosensors exhibit favorable selectivity and repeatability. The platform of all-CNT TFT biosensors incorporated with TDNs has great potential for multiplexed detection of various cancer biomarkers, providing a simple yet high performance universal strategy for low-cost clinical applications.

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基于四面体DNA纳米结构的全碳纳米管晶体管循环肿瘤DNA检测生物传感器

采用碳纳米管(CNT)晶体管作为生物传感器，具有良好的灵敏度和选择性，是一种很有前途的癌症生物标志物检测方法。然而，基于碳纳米管晶体管的生物传感器检测循环肿瘤DNA (ctDNA)仍然是一个挑战，没有任何工作报道。本研究首次提出并实现了结合四面体DNA纳米结构(TDNs)的全碳纳米管薄膜晶体管(TFT)生物传感器对三阴性乳腺癌相关的AKT2基因的直接无标记DNA检测。由于DNA杂交效率的提高，与单链DNA (ssDNA)探针相比，tdn的采用使生物传感器的响应率提高了至少35%，甚至高达98%。tdn的连接体长度对生物传感器性能的影响是非常重要的，并且已经被研究过。浓度依赖性DNA检测是由全碳纳米管TFT生物传感器实现的，具有6个数量级的宽线性检测范围和2 fM的理论检测极限。此外，全碳纳米管TFT生物传感器具有良好的选择性和可重复性。结合tdn的全碳纳米管TFT生物传感器平台具有多种癌症生物标志物的多路检测潜力，为低成本的临床应用提供了一种简单而高性能的通用策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.