[Development of an enzyme immunoassay for determination of factor VIII antigens].

Abstract

We describe an enzyme-immunoassay for the determination of factor VIII related antigen. The principle of the method is the following: Test plasma is mixed with rabbit antibodies against human factor VIII in excess and incubated at 37 degrees C. The incubation mixture is added to polystyrene tubes coated with human factor VIII. The rabbit antibody is available to adhere to factor VIII coating the tube and can be detected with an enzyme-labeled wether antibody to rabbit IgG. This method is sensitive to 7.8 x 10(-3) U/ml factor VIII antigen.