{"title":"Clinico-hematologic and serological comparison of Japanese and Russian strains of Theileria sergenti.","authors":"T Minami, T Fujinaga, K Furuya, T Ishihara","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The Japanese and Russian strains of Theileria sergenti were compared clinico-hematologically and serologically. They were subjected to passage in splenectomized calves 4 approximately 8 months old by the infectation of infected ticks or the inoculation of infected blood. As a result, the average of incubation of the Japanese strain was proved to be 23 days by the tick infection and 21 days by the inoculation of infected blood stored in a frozen state. The rate of detection and the average size of various forms of Theileria in the proliferative stage of the Japanese strain were as follows: Comma-shaped and bacilli forms, 86.8%; bacilli from 2.15 x 0.73 micron; oval and spherical forms, 11.3% and 1.97 x 1.22 and 1.46 micron, respectively; tetracoccal form and anaplasma-like form, 0.9 and 1.0%, respectively, and 0.54 micron in diameter. The highest parasitic rate of the Japanese strain was 11.9% on the average, and the rate of decrease in erythrocyte count 70.6% on the average. Rods were seen in protozoa particularly rich in cytoplasm. Such symptoms as pyrexia, depression, and anorexia appeared as Theileria propagated. No hemoglobinuria occurred or no schizonts were detected. Essentially the same results were obtained from the Russian strain as from the Japanese. Besides, Haemaphysalis longicornis produced in Japan acted also as a vector for the Russian strain. There was a good serological agreement between the Japanese and the russian strain in the results of the cross complement fixation test and the cross indirect fluorescent antibody test. There was no difference in the results of the complement fixation with the eight strains of the Japanese strain.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"20 2","pages":"44-52"},"PeriodicalIF":0.0000,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"National Institute of Animal Health quarterly","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
The Japanese and Russian strains of Theileria sergenti were compared clinico-hematologically and serologically. They were subjected to passage in splenectomized calves 4 approximately 8 months old by the infectation of infected ticks or the inoculation of infected blood. As a result, the average of incubation of the Japanese strain was proved to be 23 days by the tick infection and 21 days by the inoculation of infected blood stored in a frozen state. The rate of detection and the average size of various forms of Theileria in the proliferative stage of the Japanese strain were as follows: Comma-shaped and bacilli forms, 86.8%; bacilli from 2.15 x 0.73 micron; oval and spherical forms, 11.3% and 1.97 x 1.22 and 1.46 micron, respectively; tetracoccal form and anaplasma-like form, 0.9 and 1.0%, respectively, and 0.54 micron in diameter. The highest parasitic rate of the Japanese strain was 11.9% on the average, and the rate of decrease in erythrocyte count 70.6% on the average. Rods were seen in protozoa particularly rich in cytoplasm. Such symptoms as pyrexia, depression, and anorexia appeared as Theileria propagated. No hemoglobinuria occurred or no schizonts were detected. Essentially the same results were obtained from the Russian strain as from the Japanese. Besides, Haemaphysalis longicornis produced in Japan acted also as a vector for the Russian strain. There was a good serological agreement between the Japanese and the russian strain in the results of the cross complement fixation test and the cross indirect fluorescent antibody test. There was no difference in the results of the complement fixation with the eight strains of the Japanese strain.
采用临床血液学和血清学方法比较了日本和俄罗斯两株瑟氏杆菌。它们通过感染受感染的蜱或接种受感染的血液,在大约8个月大的去脾小牛中通过。结果表明,日本菌株经蜱虫感染的平均潜伏期为23天,经冷冻保存的感染血液接种的平均潜伏期为21天。日本株在增殖阶段,各形态的希氏菌检出率和平均大小分别为:逗号形和杆菌形,占86.8%;芽孢杆菌从2.15 x 0.73微米;椭圆形和球形,分别为11.3%和1.97 x 1.22和1.46微米;四球菌型和无原体样型,分别为0.9%和1.0%,直径0.54微米。日本菌株的最高寄生率平均为11.9%,红细胞计数平均下降率为70.6%。杆状体见于细胞质丰富的原生动物。随着芽孢杆菌的传播,出现发热、抑郁、厌食等症状。未发现血红蛋白尿或精神分裂。从俄国菌株得到的结果和从日本菌株得到的结果基本相同。此外,日本生产的长角血蜱也是俄罗斯菌株的载体。日本株和俄罗斯株的交叉补体固定试验和交叉间接荧光抗体试验结果具有良好的血清学一致性。8株日本菌株的补体固定结果无明显差异。