Protective effects of Lactiplantibacillus pentosus CQZC01 on alcohol-induced gastric mucosa injury in mice via an anti-oxidative mechanism

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods Pub Date : 2023-08-01 DOI:10.1016/j.jff.2023.105644

Ya Wu , Pan Wang , Xingyao Long , Xin Zhao

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Abstract

Gastric ulcer is a very common disease of the digestive system, and it is important to understand how this disease can be prevented and treated. In this study, the effects of Lactiplantibacillus pentosus CQZC01 (LP-CQZC01) on alcohol-induced gastric mucosal injury were investigated by evaluating the extent of hemorrhage and edema, as well as cell necrosis and gastric mucosal cell shedding. In addition, the levels of oxidative stress-related indicators, gastric mucosal defense factors, and pro-inflammatory cytokines were measured. After treatment with LP-CQZC01, the area of gastric mucosal damage was significantly reduced, and the ulcer inhibition rate and the extent of gastric mucosal damage were improved. LP-CQZC01 also significantly increased the levels of somatostatin (SS) and prostaglandin E2 (PGE2), as well as the levels of superoxide dismutase (SOD), glutathione (GSH), and glutathione peroxidase (GSH-Px); however, it decreased the malondialdehyde (MDA) content, thereby improving the anti-oxidant capacity. In addition, the levels of pro-inflammatory cytokines, such as interleukin (IL)-1 beta, IL-6, and tumor necrosis factor-alpha (TNF-α), were decreased, and the anti-inflammatory factor IL-10 was elevated. The results of polymerase chain reactions revealed that LP-CQZC01 activated the nuclear factor E2-related factor 2 (Nrf2) signaling pathway, thereby increasing the levels of downstream genes γ-glutamyl cysteine synthetase (γ-GCS), SOD, catalase (CAT), and GSH-Px. In summary, LP-CQZC01 can alleviate alcohol-induced gastric mucosal damage by increasing the levels of gastric mucosal defense factors, inhibiting oxidative stress, and suppressing inflammation. Therefore, LP-CQZC01 is a potential therapeutic agent for the prevention and treatment of alcohol-induced gastric mucosal injury.

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乳酸菌CQZC01通过抗氧化机制对酒精性小鼠胃黏膜损伤的保护作用

胃溃疡是一种非常常见的消化系统疾病，了解如何预防和治疗这种疾病非常重要。本研究通过评价胃粘膜细胞出血、水肿程度、细胞坏死和脱落情况，探讨了乳酸杆菌CQZC01 (LP-CQZC01)对酒精性胃黏膜损伤的影响。测定氧化应激相关指标、胃黏膜防御因子、促炎因子水平。经LP-CQZC01治疗后，胃黏膜损伤面积明显减少，溃疡抑制率和胃黏膜损伤程度均有改善。LP-CQZC01还显著提高了生长抑素(SS)和前列腺素E2 (PGE2)水平，以及超氧化物歧化酶(SOD)、谷胱甘肽(GSH)和谷胱甘肽过氧化物酶(GSH- px)水平;降低丙二醛(MDA)含量，提高抗氧化能力。此外，促炎因子如白细胞介素(IL)-1 β、IL-6、肿瘤坏死因子α (TNF-α)水平降低，抗炎因子IL-10水平升高。聚合酶链反应结果显示，LP-CQZC01激活核因子e2相关因子2 (Nrf2)信号通路，从而提高下游基因γ-谷氨酰半胱氨酸合成酶(γ-GCS)、SOD、过氧化氢酶(CAT)、GSH-Px水平。综上所述，LP-CQZC01可通过提高胃黏膜防御因子水平、抑制氧化应激、抑制炎症反应等方式减轻酒精性胃黏膜损伤。因此，LP-CQZC01是预防和治疗酒精性胃粘膜损伤的潜在治疗药物。

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来源期刊

Journal of Functional Foods FOOD SCIENCE & TECHNOLOGY-

CiteScore

9.60

自引率

1.80%

发文量

428

审稿时长

76 days

期刊介绍： Journal of Functional Foods continues with the same aims and scope, editorial team, submission system and rigorous peer review. We give authors the possibility to publish their top-quality papers in a well-established leading journal in the food and nutrition fields. The Journal will keep its rigorous criteria to screen high impact research addressing relevant scientific topics and performed by sound methodologies. The Journal of Functional Foods aims to bring together the results of fundamental and applied research into healthy foods and biologically active food ingredients. The Journal is centered in the specific area at the boundaries among food technology, nutrition and health welcoming papers having a good interdisciplinary approach. The Journal will cover the fields of plant bioactives; dietary fibre, probiotics; functional lipids; bioactive peptides; vitamins, minerals and botanicals and other dietary supplements. Nutritional and technological aspects related to the development of functional foods and beverages are of core interest to the journal. Experimental works dealing with food digestion, bioavailability of food bioactives and on the mechanisms by which foods and their components are able to modulate physiological parameters connected with disease prevention are of particular interest as well as those dealing with personalized nutrition and nutritional needs in pathological subjects.