The cellular substrate: a very important requirement for baculovirus in vitro replication.

H G Miltenburger, W L Naser, J P Harvey, J Huber, A M Huger
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引用次数: 27

Abstract

We established more than 200 primary cell lines of Cydia pomonella (coding moth). 81 of them were selected and screened for replication of two baculoviruses (from two different subgroups): the Choristoneura murinana NPV and the Cydia pomonella GV. Although all these cell lines had been derived from the same insect species, they varied largely in their response to challenge with the NPV. Most of them showed CPE or produced different amounts of polyhedra. Interestingly, we also found a few cell lines that were permissive for GV replication. To our knowledge this is the first time that GV replication in cell lines has been obtained. Our results show that cell line properties are most important for baculovirus in vitro replication.

细胞底物:杆状病毒体外复制的一个非常重要的条件。
建立了200多个编码蛾原代细胞系。从中选择81个样本,筛选两种杆状病毒(来自两个不同亚群)的复制情况:murinana chistoneura NPV和Cydia pomonella GV。尽管所有这些细胞系都来自同一昆虫物种,但它们对NPV挑战的反应存在很大差异。它们大多表现为CPE或产生不同数量的多面体。有趣的是,我们还发现了一些允许GV复制的细胞系。据我们所知,这是第一次在细胞系中获得GV复制。我们的研究结果表明,细胞系特性对杆状病毒的体外复制至关重要。
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