A ‘hidden line’ algorithm for 3D-reconstruction from serial sections — An extension of the NEUREC program package for a microcomputer

Abstract

An algorithm is described to generate pictures in arbitrary perspective from serially sectioned biological material. Only those parts of the object which lie on a direct line of sight to the observer are included into the computed paper-plot, while hidden lines are omitted. The program was written for an inexpensive Apple II⁺ microcomputer as an extension of an earlier program library (NEUREC) for three-dimensional reconstruction. Application examples of neuronal and cardiac tissues are presented.