Complement components in 100 newborns and their mothers determined by electroimmunoassay.

U Johnson, L Truedsson, B Gustavii
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Abstract

Samples of blood were obtained from 100 healthy full-term women in labour and, after delivery, from the umbilical cord of their infants. By electroimmunoassay, complement components were quantitated in serum (C1q, C1r, C1s, C1 IA, C2, P, D. I, H, C6 and C7) or in EDTA-plasma (C4, C3, B, C5. and C). The concentrations of C7 in cord serum was twice that found by others using a functional assay. Concentrations of C1r, I and C6 in the cord sample were 50-60 per cent of those in healthy blood donors used as reference, and that of D was about 130 per cent. The cord serum and plasma concentrations of the remaining components agreed with previously reported values. The maternal levels of C2, C4, C3, B, H, C5 were 40-60 per cent higher than those of the reference.

用电免疫分析法测定100例新生儿及其母亲的补体成分。
从100名健康的足月分娩妇女和分娩后从其婴儿的脐带中提取血液样本。采用电免疫法测定血清(C1q、C1r、C1s、C1 IA、C2、P、d I、H、C6和C7)或edta血浆(C4、C3、B、C5)中的补体成分。C).脐带血清中C7的浓度是其他人用功能测定法发现的两倍。脐带样本中C1r、I和C6的浓度是作为参考的健康献血者的50- 60%,D的浓度约为130%。脐带血清和血浆中其余成分的浓度与先前报告的值一致。产妇C2、C4、C3、B、H、C5水平较对照组高40 ~ 60%。
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