Cloning of the mouse satellite DNA.

Abstract

A stable recombinant clone was constructed by inserting a 1.5 kb mouse satellite DNA HindIII restriction fragment into the plasmid pBR-322. The cloned fragment according to both hybridisation data and restriction analysis seems to be identical with the major component of the mouse satellite DNA. It contains two Atu4001 (EcoRII) monomers, one dimer and one "1.5-mer". HindIII restriction sites are either in position around 95 or 140 of the Atu4001 monomer. Our results and the recently published prototype sequence of the mouse satellite DNA Sau961 monomer (15) suggest that HindIII cleavage of the mouse satellite DNA follows the B type restriction pattern.