Effect of ions on alpha-amylase activity of human granulocytes.

Abstract

The effect of the salts NaCl, KCl, CsCl, NH4Cl, NaBr, CH3COONa, NaNO3 and Na2CO3 on the alpha-amylase activity of dialysed and nondialysed human granulocyte extracts was measured. The optimum activity was found at the 0.02 M salt concentrations. The highest enzyme activity was found with NaCl, CsCl, NH4Cl and KCl. The apoamylase was successfully reactivated with the cations Ca2+, Ba2+, Mn2+, while reactivations with Co2+, Ni2+, Mg2+ are below the limits of significance.