{"title":"Dissociation and characterization of pilin isolated from Pseudomonas aeruginosa strains PAK and PAO.","authors":"T H Watts, C M Kay, W Paranchych","doi":"10.1139/o82-110","DOIUrl":null,"url":null,"abstract":"<p><p>Pili isolated from Pseudomonas aeruginosa strains PAK and PAO have been dissociated into subunits using the detergent octyl glucoside. Circular dichroism studies indicated that no change in protein conformation occurs as a result of this treatment. Ultracentrifugation measurements showed that both pilins have a Stokes radius of 21 A (1 A = 0.1 nm) corresponding to an axial ratio of between 3:1 and 5:1, when approximated as prolate or oblate ellipsoids. Sedimentation equilibrium measurements show that even at low protein concentrations the pilin-detergent complex exists as a mixture of monomers and dimers.</p>","PeriodicalId":9508,"journal":{"name":"Canadian journal of biochemistry","volume":"60 9","pages":"867-72"},"PeriodicalIF":0.0000,"publicationDate":"1982-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/o82-110","citationCount":"34","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Canadian journal of biochemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1139/o82-110","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 34
Abstract
Pili isolated from Pseudomonas aeruginosa strains PAK and PAO have been dissociated into subunits using the detergent octyl glucoside. Circular dichroism studies indicated that no change in protein conformation occurs as a result of this treatment. Ultracentrifugation measurements showed that both pilins have a Stokes radius of 21 A (1 A = 0.1 nm) corresponding to an axial ratio of between 3:1 and 5:1, when approximated as prolate or oblate ellipsoids. Sedimentation equilibrium measurements show that even at low protein concentrations the pilin-detergent complex exists as a mixture of monomers and dimers.
从铜绿假单胞菌菌株PAK和PAO中分离得到的毛菌用洗涤剂辛基葡萄糖苷分离成亚基。圆二色性研究表明，没有改变蛋白质构象发生作为这种处理的结果。超离心测量表明，当近似为长条形或扁圆形椭球时，两个柱状体的Stokes半径为21 a (1 a = 0.1 nm)，轴比为3:1 ~ 5:1。沉降平衡测量表明，即使在低蛋白质浓度的pilin-洗涤剂复合物存在作为单体和二聚体的混合物。
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