Isolation and identification of the glycosaminoglycans from fracture callus

Abstract

The glycosaminoglycans in fracture callus from rabbits have been isolated, and analysed with chemical and physical methods. Hyaluronic acid as well as chondroitin sulphate with infrared characteristics of both -4-sulphate and -6-sulphate were found. When callus of different degrees of maturation was studied, differences in the recovery of the total glycosaminoglycans as well as differences in the ratio between the recovered fractions were found, probably reflecting the different morphology of fracture callus of different ages.