Advanced microfluidic technologies for isolating extracellular vesicles

IF 11.8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2022-12-01 DOI:10.1016/j.trac.2022.116817

Shaohua Zhang , Jinqi Deng , Jianbin Li , Fei Tian , Chao Liu , Luo Fang , Jiashu Sun

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引用次数: 9

Abstract

Extracellular vesicles (EVs) are lipid membrane-enclosed, cell-secreted nanovesicles that have attracted significant attentions in recent years in view of their potential as non-invasive cancer diagnostic biomarkers. However, isolation and analysis of EVs from biofluids remain a challenging field due to their small size, heterogeneous marker expression, and co-existence with non-vesicular contaminants. Microfluidic techniques provide a promising approach for isolating and analyzing EVs with advantages of small sample consumption, precise fluid control, high resolution and yield, short processing time, and cost effectiveness. In this review, we summarize recent efforts in developing label-free and affinity-based microfluidic methods for isolation of EVs according to their physical and biological characteristics, and their applications in cancer diagnostics. We conclude this review with key challenges and directions for microfluidic methods for EV isolation and analysis in clinical settings.

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分离细胞外囊泡的先进微流体技术

细胞外囊泡(EVs)是一种脂质膜封闭的细胞分泌纳米囊泡，近年来由于其作为非侵入性癌症诊断生物标志物的潜力而引起了人们的广泛关注。然而，从生物体液中分离和分析电动汽车仍然是一个具有挑战性的领域，因为它们体积小，标记物表达不均，并且与非泡状污染物共存。微流控技术具有样品消耗量小、流体控制精确、分辨率和产率高、处理时间短、成本效益高等优点，为ev的分离和分析提供了一种很有前景的方法。本文从ev的物理和生物学特性及其在癌症诊断中的应用等方面，综述了近年来无标记和基于亲和的微流控分离ev的研究进展。最后，我们总结了微流体方法在临床环境中分离和分析EV的主要挑战和方向。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.