Suppression of lipid synthesis in cultured aortic cells by laminaran sulfate

Abstract

• (1)

  Sodium [1-¹⁴C]acetate was sextensively incorporated into the lipid fractions of chick aortic cells in secondary cultures maintained as monolayers, indicating a linear relation between the incorporation rate and the incubation time over 8 h.

• (2)

  When laminaran sulfate was added to this culture system, at concentrations between 2.5 and 40 μg/ml culture medium for 24–72 h, it depressed lipid synthesis in aortic cells, as measured by [¹⁴C]acetate incorporation rates into the lipid fraction.

• (3)

  Inhibition of lipid synthesis depended on the concentration of laminaran sulfate and its incubation time; inhibition was greater in the neutral fat and free sterol fractions than in the phospholipid fraction.