Optimizing the sensing performance of amperometric creatinine detection based on creatinine deiminase/Nafion®-nanostructured polyaniline composite film by mixture design method

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Jing-Shan Do, Yu-Hsuan Chang
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引用次数: 3

Abstract

Nafion®-nanostructured polyaniline (nsPANi) composite film is prepared using cyclic voltammetry (CV) and immobilized with creatinine deiminase (CD) enzyme and is used to sense creatinine in a buffer phosphate solution. The conditions for preparing Nafion®-nsPANi composite film are optimized by using a mixture design for which the sensitivity is the response. The relationship between the sensitivity of the amperometric creatinine biosensor (y) and the normalized aniline concentration (Y1), HCl concentration (Y2) and scanning rate (Y3) is y = 119.44Y1 + 45.23Y2 + 100.93Y3 + 255.69Y1Y2 + 313.16Y1Y3 + 430.56Y1Y2Y3

The maximum sensitivity of an amperometric creatinine biosensor that is constructed using Nafion®-nsPANi composite film in 0.0943 M aniline, 0.9024 M HCl and using a scanning rate of 27.88 mV s  1 is 2013.2 μA mM−1 cm−2, which is 54.9% better than the sensitivity of a conventional experimental technique. The amperometric creatinine biosensor is 6.60% less sensitive after sensing 0.15 mM creatinine 240 times. The amperometric creatinine biosensor incurs insignificant interference in 0.138 mM urea, 0.085 mM ascorbic acid (AA) and 5.54 mM glucose.

Abstract Image

采用混合设计法优化基于肌酸酐脱亚胺酶/Nafion®纳米聚苯胺复合膜的安培肌酸酐检测性能
采用循环伏安法(CV)制备了纳米结构聚苯胺(nsPANi)复合膜,并用肌酸酐脱亚胺酶(CD)酶固定,用于缓冲磷酸盐溶液中肌酸酐的检测。采用灵敏度为响应的混合设计优化了制备Nafion®-nsPANi复合膜的条件。在0.0943 M苯胺,0.9024 M HCl,扫描速率为27.88 mV s−1时,采用Nafion®-nsPANi复合膜构建的电流型肌酐生物传感器的最大灵敏度为2013.2 μA mM−1 cm−2。比传统实验技术的灵敏度提高54.9%。安培型肌酐生物传感器检测0.15 mM肌酐240次后,灵敏度降低6.60%。在0.138 mM尿素、0.085 mM抗坏血酸(AA)和5.54 mM葡萄糖中,电流型肌酐生物传感器的干扰不显著。
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来源期刊
CiteScore
9.60
自引率
0.00%
发文量
60
审稿时长
49 days
期刊介绍: Sensors and Actuators Reports is a peer-reviewed open access journal launched out from the Sensors and Actuators journal family. Sensors and Actuators Reports is dedicated to publishing new and original works in the field of all type of sensors and actuators, including bio-, chemical-, physical-, and nano- sensors and actuators, which demonstrates significant progress beyond the current state of the art. The journal regularly publishes original research papers, reviews, and short communications. For research papers and short communications, the journal aims to publish the new and original work supported by experimental results and as such purely theoretical works are not accepted.
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