Kaempferol relieves the DSS-induced chronic colitis in C57BL/6J mice, alleviates intestinal angiogenesis, and regulates colonic microflora structure

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods Pub Date : 2023-08-01 DOI:10.1016/j.jff.2023.105646

Ruyang Yu , Qilyu Zhou , Tianlong Liu , Ping Liu , Hong Li , Yifei Bian , Zhongjie Liu

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引用次数: 1

Abstract

Purpose

The progression of chronic colitis is accompanied by an increase in inflammation and intestinal angiogenesis. Kaempferol, a dietary flavonoid, has been shown to exhibit anti-inflammatory and anti-angiogenic properties. However, the anti-angiogenic effect of kaempferol in chronic colitis has not been elucidated. The present study aimed to investigate the effects of kaempferol on intestinal angiogenesis and microflora of mice with chronic colitis.

Methods

The dextran sulfate sodium (DSS)-induced chronic colitis mouse model was used in this study. The effects of kaempferol and 5-aminosalicylic acid (5-ASA) were evaluated by histopathology, ELISA, western blot, and immunohistochemistry. The integrity of the gut-vascular barrier (GVB) was studied using FITC-dextran and D-Lactate. The colonic flora of mice was analyzed using 16S rDNA sequencing.

Results

Kaempferol supplementation could relieve the symptoms (weight loss, colonic pathological injury, hemogram index, etc.) of chronic colitis in mice, down-regulate the secretion of inflammatory factors (IL-1β, IL-6, TNF-α, and CRP), and modulate the expression of inflammatory regulatory proteins (COX-2, TRAF6, and IL-22BP) in colonic tissues. Moreover, kaempferol decreased the secretion of colonic VEGF and NO, high intestinal microvascular density (MVD), and abnormal expression of VEGFR2 caused by DSS. Our finding also indicated that kaempferol alleviates chronic colitis by maintaining GVB, suppressing pro-inflammatory flora (Verrucomicrobiota, Campilobacterota, and Desulfobacterota), and enhancing the abundance of beneficial flora (Actinobacteria, and Odoribacter).

Conclusion

Kaempferol exerted its therapeutic effect on chronic colitis by alleviating the inflammation of the colon and intestinal angiogenesis, maintaining the integrity of GVB, and regulating colonic microflora. Our study demonstrated similar pharmacological effects of kaempferol to 5-ASA (drug of clinical application for intestinal inflammatory diseases), and revealed the potential of kaempferol treatment as a preventive strategy for chronic colitis.

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山奈酚可减轻dss诱导的C57BL/6J小鼠慢性结肠炎，促进肠道血管生成，调节结肠菌群结构

目的慢性结肠炎的发展伴随着炎症和肠道血管生成的增加。山奈酚，一种膳食类黄酮，已被证明具有抗炎和抗血管生成的特性。然而，山奈酚在慢性结肠炎中的抗血管生成作用尚未被阐明。本研究旨在探讨山奈酚对慢性结肠炎小鼠肠道血管生成和菌群的影响。方法采用葡聚糖硫酸钠(DSS)诱导的慢性结肠炎小鼠模型。采用组织病理学、ELISA、免疫印迹和免疫组织化学方法评价山奈酚和5-氨基水杨酸(5-ASA)的作用。采用fitc -葡聚糖和d -乳酸研究肠血管屏障(GVB)的完整性。采用16S rDNA测序对小鼠结肠菌群进行分析。结果补充山奈酚可减轻小鼠慢性结肠炎的症状(体重减轻、结肠病理损伤、血象指数等)，下调炎症因子(IL-1β、IL-6、TNF-α、CRP)的分泌，调节结肠组织中炎症调节蛋白(COX-2、TRAF6、IL-22BP)的表达。山奈酚降低了结肠VEGF和NO的分泌，提高了肠道微血管密度(MVD)，降低了DSS引起的VEGFR2的异常表达。我们的研究结果还表明，山奈酚通过维持GVB、抑制促炎菌群(Verrucomicrobiota, Campilobacterota，和Desulfobacterota)和增加有益菌群(放线菌群，Odoribacter)的丰度来缓解慢性结肠炎。结论山奈酚对慢性结肠炎的治疗作用主要体现在减轻结肠炎症和肠道血管生成、维持GVB完整性、调节结肠菌群等方面。我们的研究表明山奈酚与5-ASA(临床应用于肠道炎症性疾病的药物)的药理作用相似，揭示了山奈酚治疗作为慢性结肠炎预防策略的潜力。

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来源期刊

Journal of Functional Foods FOOD SCIENCE & TECHNOLOGY-

CiteScore

9.60

自引率

1.80%

发文量

428

审稿时长

76 days

期刊介绍： Journal of Functional Foods continues with the same aims and scope, editorial team, submission system and rigorous peer review. We give authors the possibility to publish their top-quality papers in a well-established leading journal in the food and nutrition fields. The Journal will keep its rigorous criteria to screen high impact research addressing relevant scientific topics and performed by sound methodologies. The Journal of Functional Foods aims to bring together the results of fundamental and applied research into healthy foods and biologically active food ingredients. The Journal is centered in the specific area at the boundaries among food technology, nutrition and health welcoming papers having a good interdisciplinary approach. The Journal will cover the fields of plant bioactives; dietary fibre, probiotics; functional lipids; bioactive peptides; vitamins, minerals and botanicals and other dietary supplements. Nutritional and technological aspects related to the development of functional foods and beverages are of core interest to the journal. Experimental works dealing with food digestion, bioavailability of food bioactives and on the mechanisms by which foods and their components are able to modulate physiological parameters connected with disease prevention are of particular interest as well as those dealing with personalized nutrition and nutritional needs in pathological subjects.

文献相关原料

公司名称	产品信息	采购帮参考价格
上海源叶	Kaempferol	￥35.00~￥15510.00