High internal phase Pickering emulsions stabilized by modified sturgeon myofibrillar protein for quercetin delivery

IF 3.5 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

ACS Chemical Biology Pub Date : 2023-06-07 DOI:10.1016/j.foodhyd.2023.108926

Shi-ke Shen , Yue-wen Chen , Wen-tao Yu , Qian-yun Bu , Jing-jing Fu , Zi-yi Pan , Yi-tong Wang

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引用次数: 2

Abstract

In this study, the modified sturgeon myofibrillar protein was prepared by the combination of malondialdehyde and ultrasonic induction and used as the emulsifier to form a stable high internal phase Pickering emulsion (HIPPEs) with internal phase up to 75%. The results showed that combined modification reduced the fluorescence intensity, enhanced the surface hydrophobicity, changed the secondary structure, and made the protein particles smooth. The confocal laser scanning microscopy confirmed the formation of a stable HIPPEs. The interfacial tension and rheological properties of protein stabilized HIPPEs were improved by the combined modification and the encapsulation efficiency of quercetin reached 89.32%. In vitro digestion showed that the combined modified protein-stabilized HIPPEs significantly improved the rate of lipid digestion and the bioavailability of quercetin while reducing the degradation of quercetin. The findings indicated that the utilization of modified sturgeon myofibrillar protein to stabilize HIPPEs hold significant promise for various biological applications, owing to its capacity as a carrier for the regulated discharge of bioactive agents.

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用改性鲟鱼肌原纤维蛋白稳定高内相皮克林乳剂，用于传递槲皮素

本研究采用丙二醛与超声诱导相结合的方法制备了改性鲟鱼肌原纤维蛋白，并以其为乳化剂，形成了内相高达75%的稳定高内相Pickering乳液(hipes)。结果表明，复合修饰降低了荧光强度，增强了表面疏水性，改变了二级结构，使蛋白质颗粒变得光滑。共聚焦激光扫描显微镜证实形成了稳定的hipes。复合修饰后的蛋白稳定hipes的界面张力和流变性能得到改善，槲皮素包封率达到89.32%。体外消化实验表明，联合修饰的蛋白稳定hipes显著提高了槲皮素的脂质消化率和生物利用度，同时降低了槲皮素的降解。研究结果表明，利用改性鲟鱼肌纤维蛋白来稳定hipes，由于其作为生物活性物质调节排放的载体的能力，在各种生物学应用中具有重要的前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Chemical Biology 生物-生化与分子生物学

CiteScore

7.50

自引率

5.00%

发文量

353

审稿时长

3.3 months

期刊介绍： ACS Chemical Biology provides an international forum for the rapid communication of research that broadly embraces the interface between chemistry and biology. The journal also serves as a forum to facilitate the communication between biologists and chemists that will translate into new research opportunities and discoveries. Results will be published in which molecular reasoning has been used to probe questions through in vitro investigations, cell biological methods, or organismic studies. We welcome mechanistic studies on proteins, nucleic acids, sugars, lipids, and nonbiological polymers. The journal serves a large scientific community, exploring cellular function from both chemical and biological perspectives. It is understood that submitted work is based upon original results and has not been published previously.