Direct quantification of cytosolic delivery of drug nanocarriers using FlAsH-EDT2

IF 4.7 4区 医学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
R. Rotem PhD , J.A. Bertolini PhD , L. Salvioni PhD , L. Barbieri MSc , M.A. Rizzuto PhD , V. Tinelli MSc , A. Gori PhD , S. Adams PhD , M. Colombo PhD , D. Prosperi PhD
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引用次数: 0

Abstract

The delivery of therapeutics across the cell membrane and into the cytoplasm is a major challenge that limits the development of new therapies. This challenge is compounded by the lack of a general assay for cytosolic delivery. Here we develop this assay based on the pro-fluorophore CrAsH-EDT2, and provide cytosolic penetration results for a variety of drug delivery agents (polyethyleneimine, poly-arginine, Ferritin, poly [maleic anhydride-alt-isobutene] grafted with dodecylamine, and cationic liposomes) into HeLa and T98G cells. Our results show that this method can be widely applicable to different cells and drug delivery agents, and yield statistically robust results. We later use this method to optimize and improve a model drug delivery agent's (Ferritin) cytosolic penetration.

Abstract Image

使用FlAsH-EDT2直接定量药物纳米载体的胞内递送
药物跨越细胞膜进入细胞质是限制新疗法发展的主要挑战。由于缺乏细胞质输送的通用检测方法,这一挑战更加严峻。在此,我们基于前荧光团CrAsH-EDT2开发了这项检测,并提供了各种药物递送剂(聚乙烯亚胺、聚精氨酸、铁蛋白、接枝十二胺的聚马来酸酐-异丁烯和阳离子脂质体)进入HeLa和T98G细胞的细胞质渗透结果。我们的结果表明,该方法可以广泛适用于不同的细胞和药物递送剂,并产生统计稳健的结果。我们随后用这种方法优化和改进了一种模型药物递送剂(铁蛋白)的细胞质渗透。
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来源期刊
CiteScore
8.10
自引率
3.60%
发文量
104
审稿时长
4.6 months
期刊介绍: Nanomedicine: Nanotechnology, Biology and Medicine (NBM) is an international, peer-reviewed journal presenting novel, significant, and interdisciplinary theoretical and experimental results related to nanoscience and nanotechnology in the life and health sciences. Content includes basic, translational, and clinical research addressing diagnosis, treatment, monitoring, prediction, and prevention of diseases.
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