Time-lapse microcinematography of the effect of thiazolidine carboxylic acid (thioproline) upon tissue cultures.

Revista espanola de oncologia Pub Date : 1985-01-01
Y Alvarez-Rodríguez, A Ruano, J Alvarez-Noves, Y Valladares
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Abstract

This work deals with the effect of thiazolidine-4-carboxylic acid (thioproline) upon several in vitro established human cell lines. Times-lapse microcinematography is used to study the action of thioproline on HeLa cells, employing high doses during a short time, and continuous exposure to medium and small doses. The effect of thioproline in mixed cultures of HeLa cells and human lymphoblasts is described. Special attention is paid to the observed cell cycle phase specificity, and to the phenomenon described as reverse transformation that has been associated to the drug. Marked differences is the sensitivity to thioproline are observed for the different types of in vitro established cell lines. The effect is always produced during the G1 and S phases of the cell cycle. Cell changes are produced only after contacting with one another, and the biochemical reactions which are proper of cell contact inhibition of growth and/or genotypic reverse transformation were never observed, so that the mechanism of action of the drug has to be revised. From a morphodynamic point of view, thioproline is an interesting drug because it produces peculiar effects which differ markedly from those produced by the other cancer chemotherapy agents. Thioproline could prove to be useful for cancer chemotherapy if used in combination with other drugs, knowing that it is a low activity phase specific antineoplastic drug and not a drug producing reverse transformation.

噻唑烷羧酸(硫脯氨酸)对组织培养效果的延时微缩摄影。
这项工作涉及噻唑烷-4-羧酸(硫脯氨酸)对几种体外建立的人类细胞系的影响。采用延时微缩摄影技术研究硫脯氨酸对HeLa细胞的作用,采用短时间高剂量,连续暴露于中、小剂量。描述了硫脯氨酸在HeLa细胞和人淋巴母细胞混合培养中的作用。特别注意观察到的细胞周期期特异性,以及被描述为与药物相关的反向转化的现象。不同类型的体外培养细胞系对硫脯氨酸的敏感性有显著差异。这种作用总是在细胞周期的G1和S期产生。细胞相互接触后才产生变化,没有观察到细胞接触抑制生长和/或基因型逆转的生化反应,因此需要对药物的作用机制进行修正。从形态动力学的角度来看,硫脯氨酸是一种有趣的药物,因为它产生的特殊效果与其他癌症化疗药物产生的效果明显不同。硫脯氨酸是一种低活性期特异性抗肿瘤药物,而不是一种产生反向转化的药物,如果与其他药物联合使用,硫脯氨酸可能被证明对癌症化疗有用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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